AI Article Synopsis
- The study aimed to investigate the effects of the peptide Mut3DPT-PP2A/SET on the interaction between the serine threonine phosphatase PP2A and the oncoprotein SET in a breast adenocarcinoma mouse model.
- The experimental setup involved adult male mice with grafted tumors, where one group received the peptide treatment and the other a placebo, and various parameters like tumor size and histological changes were measured over a period of time.
- Results indicated that the peptide treatment led to significant tumor size reduction and observable signs of cell death in tumors, suggesting its potential as an effective anti-cancer therapy in future applications.
Article Abstract
Objective: To test cell penetrating and interfering peptide Mut3DPT-PP2A/SET in interaction between serine threonine phosphatase PP2A and its physiological inhibitor, the oncoprotein SET.
Materials And Methods: Adult male C3H/S-strain mice, 60 days old, were given a graft of breast adenocarcinoma cells (TN60) into subcutaneous tissue. Mut3DPT-PP2A/SET peptide was used to block PP2A and SET oncoprotein interaction. The graft-bearing animals were divided into a control group (injected with saline buffer), and an intervention group injected intraperitoneally with Mut3DPT-PP2A/SET peptide (5 mg/kg) every day from day 5 to day 37. The variables we used to compare the outcome in both groups were tumor size in mm (length×width) and histological changes. In the statistical analysis we used ANOVA and Student-Keuls multiple comparisons test and Tuckey for the post-test analysis.
Results: 48 mice were grafted at day 0 with breast UNLP-C3H/S tumor cells, and after randomization, they were assigned to one of the two study groups. At day 5 all mice were injected either with placebo or with the peptide. The treated group showed significant tumor reduction (p<0.07). Histological changes showed presence of apoptosis and necrosis of tumor in treated group.
Conclusion: The peptide Mut3DPT-PP2A/SET has demonstrated anti-tumor activity by reduction in vivo of tumor growth becoming a promising future in anticancer therapy.
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| http://dx.doi.org/10.3897/folmed.62.e47737 | DOI Listing |
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