Reddish purple Chinese cabbage (RPCC) is a popular variety of (AA = 20). It is rich in anthocyanins, which have many health benefits. We detected novel anthocyanins including cyanidin 3-(feruloyl) diglucoside-5-(malonoyl) glucoside and pelargonidin 3-(caffeoyl) diglucoside-5-(malonoyl) glucoside in RPCC. Analyses of transcriptome data revealed 32,395 genes including 3345 differentially expressed genes (DEGs) between 3-week-old RPCC and green Chinese cabbage (GCC). The DEGs included 218 transcription factor (TF) genes and some functionally uncharacterized genes. Sixty DEGs identified from the transcriptome data were analyzed in 3-, 6- and 9-week old seedlings by RT-qPCR, and 35 of them had higher transcript levels in RPCC than in GCC. We detected regulatory motifs of MYB, bHLH, WRKY, bZIP and AP2/ERF TFs in anthocyanin biosynthetic gene promoters. A network analysis revealed that MYB75, MYB90, and MYBL2 strongly interact with anthocyanin biosynthetic genes. Our results show that the late biosynthesis genes and and the regulatory MYB genes and are highly expressed in RPCC, indicative of their important roles in anthocyanin biosynthesis, modification, and accumulation. Finally, we propose a model anthocyanin biosynthesis pathway that includes the unique anthocyanin pigments and genes specific to RPCC.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215907 | PMC |
| http://dx.doi.org/10.3390/ijms21082901 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
"From Waste to Wonder": Comparative Evaluation of Chinese Cabbage Waste and Banana Peel Derived Hydrogels on Soil Water Retention Performance.
Gels
December 2024
College of Resources and Environment Sciences, Gansu Agricultural University, Lanzhou 730070, China.
Under the increasing severity of drought issues and the urgent need for the resourceful utilization of agricultural waste, this study aimed to compare the soil water retention properties of hydrogels prepared from Chinese cabbage waste (CW) and banana peel (BP) using grafting techniques with acrylic acid (AA) and acrylamide (AAm). Free radical polymerization was initiated with ammonium persulfate (APS), and N, N'-methylene bisacrylamide (MBA) served as the crosslinking agent to fabricate the grafted polymer hydrogels. The hydrogels were subjected to detailed evaluations of their water absorption, reusability, and water retention capabilities through indoor experiments.
View Article and Find Full Text PDF[Effects of seed carrier-immobilized microorganisms on the growth of rapeseed and the remediation of petroleum hydrocarbon contaminated soil].
Ying Yong Sheng Tai Xue Bao
October 2024
School of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, China.
Petroleum hydrocarbon pollutants in soil are challenging to biodegrade, negatively impacting plant growth as well as the metabolic activity and community structure of soil microorganisms. Microorganisms immobilized by seed carriers can synergistically contribute to the remediation of petroleum hydrocarbon-contaminated soil. We prepared a rape seed carrier with immobilized microorganism by seed coating (with a mixture of diatomaceous earth and bentonite as fillers) and microbial immobilization.
View Article and Find Full Text PDFScorpion insect neurotoxin LqhIT2 is a promising oral biopesticide: high-level preparation in Pichia pastoris and bioactivity assays.
Pest Manag Sci
December 2024
Postdoctoral Mobile Station of Biology, Genetic Engineering Research Center, College of Life Sciences, Chongqing University, Chongqing, China.
Background: Discovering insecticidal proteins with high activity and strict insect specificity and applying them to the biological control of insect pests is of great significance. Oral LqhIT2 has insecticidal activity, which most other insecticidal neurotoxin proteins do not have, but the large-scale preparation of the toxin is difficult and one of the obstacles to determining its anti-insect potential for biological control.
Results: In this study, the expression level of recombinant LqhIT2 (rLqhIT2) in Pichia pastoris was as high as 1.
Identification and expression analysis of TALE superfamily genes explore their key roles in response to abiotic stress in Brassica napus.
BMC Plant Biol
December 2024
The Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430000, China.
Background: The three-amino-acid-loop-extension (TALE) superfamily genes are broadly present in plants and play important roles in plant growth, development, and abiotic stress responses. So far, the TALE family in B.napus have not been systematically studied, especially their potential roles in response to abiotic stress.
View Article and Find Full Text PDFThe pan-NLRome analysis based on 23 genomes reveals the diversity of NLRs in .
Mol Breed
December 2024
Yazhouwan National Laboratory, Sanya, 572025 Hainan China.
, a globally significant oilseed crop, exhibits a wide distribution across diverse climatic zones. is being increasingly susceptible to distinct diseases, such as blackleg, clubroot and sclerotinia stem rot, leading to substantial reductions in yield. Nucleotide-binding site leucine-rich repeat genes (), the most pivotal family of resistance genes, can be effectively harnessed by identifying and uncovering their diversity to acquire premium disease-resistant gene resources.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!