Bacillus amyloliquefaciens, which is closely related to Bacillus subtilis, produces a series of metabolites that can inhibit the growth of fungi and bacteria. Here, we investigated the effect of B. amyloliquefaciens used as a probiotic on the innate immunity of the crayfish Procambarus clarkii when challenged with white spot syndrome virus (WSSV). Dietary B. amyloliquefaciens supplement significantly reduced the mortality of WSSV-challenged crayfish and reduced copy numbers of WSSV. The quantitative reverse transcription-polymerase chain reaction results showed that B. amyloliquefaciens supplement increased the expression of several immune-related genes, including Toll-like receptor, NF-κB and C-type-lectin. Further analysis showed that B. amyloliquefaciens supplement also had an effect on three immune parameters, including total hemocyte count, phenoloxidase activity and superoxide dismutase activity. In both infected and uninfected crayfish, B. amyloliquefaciens supplement significantly decreased hemocyte apoptosis. Our results showed that B. amyloliquefaciens can regulate innate immunity of crayfish and reduce the mortality following WSSV challenge. This study provides a novel insight into the potential for therapeutic or prophylactic intervention with B. amyloliquefaciens to regulate crayfish immunity and protect against WSSV infection, and also provides a theoretical basis for the use of probiotics as aquatic feed additives.
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http://dx.doi.org/10.1016/j.fsi.2020.04.033 | DOI Listing |
Animals (Basel)
December 2024
Faculty of Medicine Veterinary and Animal Science, Federal University of Lavras, Lavras 37200-000, MG, Brazil.
This study investigated the efficacy of using probiotics on the performance and health parameters of sows and their litters. A randomized block design was used with 584 sows and 292 replications, with two dietary treatments: the control group (basal diet without probiotics) and the probiotic group (basal diet supplemented with 400 g/ton of a probiotic composed of () 541 and () 516). Feed intake was evaluated throughout the experimental period.
View Article and Find Full Text PDFWorld J Microbiol Biotechnol
December 2024
Facultad de Ciencias Exactas, Instituto de Investigaciones para la Industria Química (INIQUI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Salta, Salta, 4400, Argentina.
Leather industry is traditionally characterized by the use of large amounts of chemical agents, some of which are toxic to human health and the environment. However, during the last years, many efforts have been made with the aim of successfully implement enzymes as agents for different leather production stages. The lipopeptides produced by the Bacillus spp.
View Article and Find Full Text PDFHeliyon
November 2024
Department of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Sharkia, Egypt.
Fish Shellfish Immunol
January 2025
Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, China; College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang, 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, 524002, China; Guangxi Key Laboratory for Marine Natural Products and Combinational Biosynthesis Chemistry, Nanning, 530200, China; Guangxi Beibu Gulf Marine Research Centre, Guangxi, Academy of Sciences, Nanning, 530007, China. Electronic address:
This research work investigated the efficacy of Bacillus amyloliquefaciens AV5 in mitigating the stress effects of stocking densities in Nile tilapia (Oreochromis niloticus). 520 healthy Nile tilapia fingerlings (24.43 ± 1.
View Article and Find Full Text PDFAquac Nutr
August 2024
College of Fishery Guangdong Ocean University, Zhanjiang 524088, China.
In the present study, we investigated the effect of dietary supplementation with the probiotic AV5 (OR647358) on the growth, serum and mucus immune responses, metabolomics, and lipid metabolism of . Fishes (27.2 ± 1.
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