A novel method for the extraction of outer membrane vesicles (OMVs) from Tohama strain.

Iran J Microbiol

Department of Human Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Published: February 2020

Background And Objectives: There are many pertussis outbreaks which is mainly due to the reduction in the immunity of acellular pertussis (aP) vaccines. Therefore, there is a crucial necessity to develop a new generation of pertussis vaccine. Preceding researches have shown that outer membrane vesicles (OMVs) have appropriate specifications, making them a suitable vaccine candidate against pertussis.

Materials And Methods: The OMVs were separated by a new serial ultra centrifugation technique. Transmission electron microscopy (TEM) examination, SDS-PAGE, Western blotting and ELISA assay were used to characterize the OMVs.

Results: TEM studies showed the size of the extracted OMVs at 40-200 nm. The presence of pertussis toxin, filamentous hemagglutinin, and pertactin was verified using Western blot and ELISA assay.

Conclusion: The presented technique is a simple and effective way to obtain OMVs from So it can be utilized as an appropriate procedure in the development of an OMV-based vaccine against pertussis.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7163034PMC

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