The aim of this study was to explore the possible protective mechanisms and to determine the antioxidant capacity of phenolic compounds extracted from against lead acetate-induced hepatic injury. High performance liquid chromatography-photo diode array/electrospray ionization-mass spectrometry (HPLC-PDA/ESI-MS) assay was used to identify the extract phenolic compounds. Animals received 100 mg of lead acetate/kg of body weight (bw) in the drinking water for a period of 30 days. The other groups of rats were orally administered with silymarin (300 mg/kg bw) or the extract at two doses (100 and 300 mg/kg of bw), once daily, by gastric gavage for the same time. The exhibited high total phenolic, flavonoid, and anthocyanin contents. The antioxidant activity demonstrated that the exhibits an important effect against deleterious reactive species. The results showed that prevented the lead acetate-induced significant changes on serum and liver lipid levels. In contrast, succeeded in improving the biochemical parameters of serum and liver bringing them closer to the normal values of the control group. It also significantly promoted ( < .05) pro-inflammatory cytokines (TNF-, IL-6, and NF-B) in the liver of the experimental animals. The evaluated sample with HPLC-PDA/ESI-MS method showed to contain 10 dominant polyphenols, 2 hydroxycinnamic acids (p-coumaric acid and chlorogenic acids), 4 flavones (Apigenin, Luteolin, Cirsiliol, and Luteolin-7-O-rutinoside), and an anthocyanin (cyanidin-3-glucoside). Hence, it can be concluded that could be a potent source of health-beneficial phytochemicals providing a novel therapy to protect liver against lead exposure.
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http://dx.doi.org/10.1089/jmf.2019.0246 | DOI Listing |
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