AI Article Synopsis
- Hypochlorous acid (HClO) is crucial in various physiological processes, and abnormal levels are linked to diseases like inflammation and cancer.
- A new probe called DDAO-ClO was developed to detect HClO through a specific reaction that allows for rapid colorimetric and near-infrared fluorescence responses.
- DDAO-ClO demonstrated high sensitivity and fast response times in vitro, and it successfully imaged HClO in living cells and animals, revealing increased HClO production in injured liver tissues.
Article Abstract
Hypochlorous acid (HClO) is an important bioactive molecule, playing vital roles in a large number of physiological and pathological processes. Abnormal concentration of HClO in vivo has close contact with many diseases including inflammatory diseases and cancer. For bioimaging HClO, a new colorimetric and turn-on near-infrared (NIR) fluorescence probe (DDAO-ClO) was designed and synthesized in this work through a specific reaction of HClO with dimethylthiocarbamate. DDAO-ClO proved to show distinct and highly selective colorimetric and NIR fluorescence responses for HClO with fast response time (<3 s) and high sensitivity (LOD = 7.3 nM) in vitro. After confirming the excellent in vitro sensing ability, imaging HClO with DDAO-ClO in living HeLa cells, MCF-7 cells, zebrafish, and mice was all successfully demonstrated. And with this probe, it was further discovered that more endogenous HClO was produced in injured mice liver tissues, which demonstrates that DDAO-ClO not only is effective for in vivo detection of HClO but also has a broad application prospect.
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| http://dx.doi.org/10.1016/j.talanta.2020.120901 | DOI Listing |
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