Molecular identification of species causing endophthalmitis using multilocus sequence analysis (MLSA): a 10-year perspective.

spp. can cause several ocular infections, such as keratitis, endophthalmitis and scleral abscesses. Molecular identification of spp. by sequencing is the gold standard method at present for species identification, but closely related species can only be identified by multilocus sequence analysis (MLSA) of housekeeping genes. The major objective was to profile species, antibiotic susceptibility patterns and clinical outcomes in endophthalmitis patients. Between January 2009 and December 2018, endophthalmitis patients who were diagnosed with infection based on microscopic and culture characteristics were selected. Antibacterial susceptibility tests were performed and speciation was performed using MLSA and phylogenetic tree analysis of the 16 s rRNA gene and the , and genes. A total of 43 culture-proven patients were identified during the study period. All isolates were 100 % sensitive to amikacin and 98 % resistant to ceftazidime. Fluoroquinolone sensitivity was observed in the range of 58 to 72 %. Year-wise analysis of antibiotic resistance patterns revealed there was a significant increase in resistance to fluoroquinolones. Twenty-two isolates were stored and six different species were identified. (=10) was found to be the most predominant, followed by (=4), (=3), (=2), (=2) and (=1). is the major pathogen, and this is the first report to identify , and as causing endophthalmitis. Overall, visual outcomes were mostly poor even after aggressive management.