Diagnostic value and clinical application of next-generation sequencing for infections in immunosuppressed patients with corticosteroid therapy.

Background: Next-generation sequencing (NGS) is a comprehensive approach for sequence-based identification of pathogens. However, reports on the use of NGS in patients with immunosuppression are scarce, especially in subjects with negative microbiological results.

Methods: In this study, NGS was performed on samples obtained from 108 anonymized patients with suspected infection undergoing immunosuppressive corticosteroid therapy. A panel of conventional microbiological tests (CMT) was performed in parallel with NGS.

Results: Of these 108 subjects, 36 were diagnosed with infections by clinical and microbiological criteria (Group I), 41 were exclusively diagnosed clinically (Group II), and 31 exhibited no evidence of infection (Group III). In Group I, NGS was concordant with CMT results from 29 patients (80.6%). A total of 4 samples had positive NGS results in Group III. NGS showed a sensitivity of 80.6% (95% CI, 64.7% to 90.6%) and specificity of 87.1% (95% CI, 70.5% to 95.5%). NGS also played an important role in optimizing antibiotic regimens in patients with negative results for CMT (Group II). The treatment success rate (TSR) of patients using NGS-guided antibiotic regimens (81.8%, 18/22) was significantly higher than that of patients using empirical antibiotics (52.6%, 10/19) (P<0.0001). NGS results were not affected by the degree of immunosuppression.

Conclusions: NGS of clinical samples from immunosuppressed patients demonstrated promising diagnostic potential in identifying clinically relevant pathogens. Consequently NGS stands to become a standard tool for infection detection and control, providing valuable information to optimize antibiotic therapy in immunosuppressed patients.