AI Article Synopsis

  • Sheeppox and goatpox are serious viral diseases affecting sheep and goats, caused by the sheeppox virus and goatpox virus, respectively, which belong to the Capripoxvirus genus.
  • Analysis of the ORF095 gene from various SPPV and GTPV isolates in India revealed highly conserved regions and distinct species-specific residues, crucial for understanding their genetic makeup.
  • The study also highlighted potential applications of the ORF095 protein for developing diagnostics and immunoassays, enhancing the ability to identify and monitor these animal diseases.

Article Abstract

Sheeppox and goatpox are important transboundary animal viral diseases of sheep and goats caused by sheeppox virus (SPPV) and goatpox virus (GTPV), respectively, of the genus Capripoxvirus, family Poxviridae. Among the proteins encoded by the capripoxvirus (CaPV) genome, ORF095 (vaccinia virus A4L homolog) is an immunodominant virion core protein that plays a pivotal role in virus assembly and morphogenesis. In the present study, sequence analysis of the ORF095 genes of 27 SPPV and GTPV isolates or field samples from different geographical regions of India was performed, and structure was prediction was done by homology modeling. A multiple sequence alignment of different CaPV isolates revealed that CaPV-A4L is highly conserved, with several species-specific signature residues, namely A93, A216, A315, G136 and G146 in GTPV, G47, A63, A168 and A276 in SPPV, and G48 and C98 in lumpy skin disease virus (LSDV). Phylogenetically, the CaPV isolates were separated into three major clusters, GTPV, SPPV and LSDV, based on the complete coding sequence of the CaPV-A4L gene. Genus-specific clustering of poxviruses was observed in phylogenetic analysis based on A4L protein homologs of chordopoxviruses. A secondary structure prediction showed the presence of six α-helices and one β-sheet as well as some coils. The signature residues identified here are potentially useful for genotyping, and the predicted characteristics of the CaPV-A4L protein make it an ideal candidate for use as an immunogenic or diagnostic antigen for the development of immunoassays in  the sero-evaluation of CaPV in target hosts.

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http://dx.doi.org/10.1007/s00705-020-04623-5DOI Listing

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Article Synopsis
  • Sheeppox and goatpox are serious viral diseases affecting sheep and goats, caused by the sheeppox virus and goatpox virus, respectively, which belong to the Capripoxvirus genus.
  • Analysis of the ORF095 gene from various SPPV and GTPV isolates in India revealed highly conserved regions and distinct species-specific residues, crucial for understanding their genetic makeup.
  • The study also highlighted potential applications of the ORF095 protein for developing diagnostics and immunoassays, enhancing the ability to identify and monitor these animal diseases.
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RNA interference targeting virion core protein ORF095 inhibits Goatpox virus replication in Vero cells.

Virol J

February 2012

Key Laboratory of Animal virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Gansu, PR China.

Background: Goatpox is an economically important disease in goat and sheep-producing areas of the world. Many vaccine strategies developed to control the disease are not yet completely successful. Hairpin expression vectors have been used to induce gene silencing in a large number of studies on viruses.

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