Tectonins are conserved defense proteins of innate immune systems featuring a β-propeller fold. Tectonin 2 from Laccaria bicolor, Lb-Tec2, is the first fungal representative of the tectonin superfamily that has been described. In-depth characterization revealed a specificity for O-methylated glycans and identified a unique sequence motif and binding site architecture underlying this unusual specificity. This chapter provides information on how to produce and purify recombinant Lb-Tec2, characterize its interaction with O-methylated glycans and demonstrate its biological function.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-0430-4_58 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Structural characterization and anti-hyperuricemic effect of a mannogalactan from Armillariella tabescens mycelium.
Int J Biol Macromol
December 2024
Anhui Key Laboratory for Ecological Engineering and Biotechnology, School of Life Sciences, Anhui University, Hefei 230601, Anhui, China. Electronic address:
Hyperuricemia and its complications caused by purine metabolism disorder continue to occur, which require effective drug treatment with fewer side effects. This study explored the positive effects of a natural homogeneous polysaccharide (AT-W) from the mycelium of Armillariella tabescens in a mouse hyperuricemia model. Structural characterization showed that the average molecular weight of AT-W is 25.
View Article and Find Full Text PDFIsorhamnetin alleviates ferroptosis-mediated colitis by activating the NRF2/HO-1 pathway and chelating iron.
Int Immunopharmacol
June 2024
Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu 214122, China; Insitute of Health and Medicine, Hefei Comprehensive National Science Center, Hefei 230601, China. Electronic address:
Ferroptosis of intestinal epithelial cells (IECs) had been identified as a key factor in the development of ulcerative colitis (UC). Therefore, targeted inhibition of ferroptosis may provide a new strategy for the treatment of UC. Isorhamnetin (ISO) was an O-methylated flavonol with therapeutic effects on a variety of diseases, such as cardiovascular disease, neurological disorders and tumors.
View Article and Find Full Text PDFStructure and Binding Properties to Blood Co-Factors of the Least Sulfated Galactan Found in the Cell Wall of the Red Alga .
Mar Drugs
February 2024
Department of BioMolecular Sciences, University of Mississippi, Oxford, MS 38677, USA.
Three different populations of sulfated polysaccharides can be found in the cell wall of the red alga . In a previous work, the structures of the two more sulfated polysaccharides were revised. In this work, NMR-based structural analysis was performed on the least sulfated polysaccharide and its chemically modified derivatives.
View Article and Find Full Text PDFMetal-binding cyclodextrins: Synthesis and complexation with Zn and Ga cations towards antimicrobial applications.
Carbohydr Polym
December 2023
Institute of Nanoscience and Nanotechnology, National Center for Scientific Research "Demokritos", Patr. Grigoriou E´ & 27 Neapoleos str., 15341, Aghia Paraskevi, Attiki, Greece. Electronic address:
Highly resistant bacteria producing metallo-β-lactamases (MBLs) to evade β-lactam antibiotics, constitute a major cause of life-threatening infections world-wide. MBLs exert their hydrolytic action via Zn cations in their active center. Presently, there are no approved drugs to target MBLs and combat the associated antimicrobial resistance (AMR).
View Article and Find Full Text PDFA regular Chlorella mannogalactan and its sulfated derivative as a promising anticoagulant: Structural characterization and anticoagulant activity.
Carbohydr Polym
August 2023
Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China; Guangxi Key Laboratory of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China. Electronic address:
Chlorella is one of the most widely cultivated species of microalgae and has been consumed as a "green healthy food". In this study, a novel polysaccharide (CPP-1) was isolated from Chlorella pyrenoidosa, structurally analyzed, and sulfated as a promising anticoagulant. Structural analyses by chemical and instrumental methods such as monosaccharide composition, methylation-GC-MS and 1D/2D NMR spectroscopy analysis revealed that CPP-1 had a molecular weight of ~13.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!