AI Article Synopsis
- The study focuses on the effectiveness of the BioFire FilmArray Blood Culture Identification (BCID) panel in quickly identifying pathogens and their antibiotic resistance in flagged blood cultures, which is crucial for timely treatment of bloodstream infections.
- A total of 100 blood cultures were analyzed and compared; the results showed a 94% consistency between the FilmArray BCID panel and traditional methods (MALDI-TOF MS and Vitek 2) for detecting pathogens and assessing their antibiotic susceptibility.
- The FilmArray BCID panel not only matched well with conventional methods but also provided faster results, especially for detecting resistant bacteria like MRSA, VRE, and carbapenem-resistant Enterobacteriaceae.*
Article Abstract
Background/purpose: Rapid and accurate identification of pathogens and their antibiotic resistance directly from flagged blood cultures can aid clinicians in optimizing early antibiotic treatment and improve the clinical outcomes, especially in settings associated with high rates of bloodstream infection caused by vancomycin-resistant Enterococci (VRE) and carbapenem-resistant Enterobacteriaceae (CRE). We compared the results of the BioFire FilmArray Blood Culture Identification (BCID) panel with those of conventional methods for identifying the pathogens and their antibiotic susceptibility status.
Methods: In total, 100 randomly selected positive blood cultures (BACTEC Plus Aerobic/F bottles or BACTEC Anaerobic Lytic/10 bottles) were analyzed. The pathogen detection efficiency of FilmArray BCID panel was compared with that of conventional method using MALDI-TOF MS system (Bruker MALDI Biotyper) and susceptibility testing by the Vitek 2 system. The sequencing analysis of antibiotic resistance genes was performed for discrepant results obtained from MALDI Biotyper and Vitek 2.
Results: Among the 100 positively flagged blood cultures, 94% of FilmArray BCID panel results were consistent with the MALDI Biotyper results. All five VRE isolates positive for vanA/vanB genes, 10 of 12 Staphylococcus species positive for mecA gene, and only one Klebsiella pneumoniae isolate positive for K. pneumoniae carbapenemase gene (bla) detected in the FilmArray BCID panel were also concordant with results by the results by conventional susceptibility testing/molecular confirmation.
Conclusions: The FilmArray BCID panel results not only demonstrated good correlation with conventional blood culture identification and susceptibility results but also provided results rapidly, especially for the early detection of MRSA, VRE and bla-mediated CRE.
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| http://dx.doi.org/10.1016/j.jmii.2020.03.018 | DOI Listing |
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