AI Article Synopsis
- Developed a MLPA assay to detect 6 viral pathogens responsible for porcine reproductive failure, including PRRSV and CSFV.
- The assay's detection limits varied from 1 to 8 copies per test for different viruses, and it was evaluated using 346 cord blood samples compared to real-time PCR results.
- The MLPA assay demonstrated high sensitivity and specificity for most viruses, showing comparable effectiveness to real-time PCR for fast screening of these pathogens.
Article Abstract
We developed a multiplex ligation-dependent probe amplification (MLPA) assay for the simultaneous detection of 6 clinically relevant viral pathogens causing porcine reproductive failure, that is porcine reproductive and respiratory syndrome virus (PRRSV), Japanese encephalitis virus (JEV), classical swine fever virus (CSFV), porcine circovirus type 2 (PCV2), pseudorabies virus (PRV) and porcine parvovirus (PPV). The limits of detection for the assay varied among the 6 target organisms from 1 to 8 copies per MLPA assay. The MLPA assay was evaluated with 346 heparinized porcine umbilical cord blood specimens, and the results of the assay were compared to those of real-time PCR. The MLPA assay showed specificities and sensitivities of 99.2% and 100%, respectively, for PRRSV; 100% and 100%, respectively, for CSFV, PCV2, PRV and PPV. No sample was found to be positive for JEV by either the MLPA assay or the real-time PCR. In conclusion, the MLPA assay has comparable clinical sensitivity to that of real-time PCR assay and provides a useful tool for fast screening porcine reproductive failure-associated viruses.
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| http://dx.doi.org/10.1111/tbed.13585 | DOI Listing |
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