MicroRNA-455-5p exerts inhibitory effect in cervical carcinoma through targeting S1PR1 and blocking mTOR pathway.

Background: MicroRNAs (miRNAs) have been increasingly exploited in human malignancies. The regulation of microRNA-455-5p (miR-455-5p) has been shown in several cancers, except for cervical carcinoma. Therefore, the role of miR-455-5p was exploited in cervical carcinoma.

Methods: The qRT-PCR experiment was used to assess miR-455-5p and S1PR1 expression levels. We explored the function of miR-455-5p through MTT and Transwell assays. The mTOR pathway and cell apoptosis were detected by Western blot assays. The relationship between miR-455-5p and S1PR1 was testified by dual-luciferase reporter assay.

Results: MiR-455-5p expression was decreased in cervical carcinoma, which was related to poor clinical outcome in cervical carcinoma patients. MiR-455-5p inhibited cell viability and metastasis in cervical carcinoma. Further, S1PR1 is a direct target of miR-455-5p. S1PR1 recovered the inhibition of cell viability and metastasis induced by miR-455-5p in cervical carcinoma. In addition, miR-455-5p induced cell apoptosis and inactivated the mTOR pathway in cervical carcinoma.

Conclusion: MiR-455-5p exerts inhibitory effect in cervical carcinoma through targeting S1PR1 and blocking the mTOR pathway.