AI Article Synopsis
- - CHRFAM7A is a human gene that acts as an inhibitor of the α7 nicotinic acetylcholine receptor and is found in human immune cells, influencing how they stick together and move.
- - The study used various human cell lines, including THP-1, to investigate the impact of CHRFAM7A on cell behavior, such as migration and growth.
- - Results indicated that adding CHRFAM7A to THP-1 cells decreases their migration and colony formation capabilities, suggesting it plays a crucial role in regulating the activity of immune cells.
Article Abstract
Objective And Design: CHRFAM7A is a unique human gene that encodes a dominant negative inhibitor of the α7 nicotinic acetylcholine receptor. We have recently shown that CHRFAM7A is expressed in human leukocytes, increases cel-cell adhesion, and regulates the expression of genes associated with leukocyte migration.
Material: Human THP-1, RAW264.7 and HEK293 cells.
Methods: Cell migration, cell proliferation and colony formation in soft agar to compare the biological activity of vector vs. CHRFAM7A-transduced cells.
Results: We show that gene delivery of CHRFAM7A into the THP-1 human monocytic cell line reduces cell migration, reduces chemotaxis to monocyte chemoattractant protein, and reduces colony formation in soft agar.
Conclusion: Taken together, the findings demonstrate that CHRFAM7A regulates the biological activity of monocytes/macrophages to migrate and undergo anchorage-independent growth in vitro.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263946 | PMC |
| http://dx.doi.org/10.1007/s00011-020-01349-7 | DOI Listing |
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