AI Article Synopsis
- The study examined how chick embryo brain cells develop under different culture conditions, focusing on morphological changes and staining for acetylcholinesterase.
- Cells from 7-day embryos showed similar development across all conditions tested.
- For 11-day embryos, dissociating cells with trypsin and using a plastic surface resulted in the best development compared to using mechanical dissection and a collagen surface.
Article Abstract
In vitro differentiation of chick embryo brain cells was compared under several culture conditions. Morphological observations and acetylcholinesterase histochemical staining revealed that the development was similar in all conditions tested if cells have been derived from 7 days embryos. Considering the cultures from 11 days embryos, the cell dissociation by trypsin and the plastic surface proved to be the most favourable conditions in contrast to mechanical dissection and collagen surface.
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