spp. is an oomycetes pathogen which causes serious damage to a wide range of crops. Bud rot disease of coconut palm, caused by , causes huge economic losses since it cannot be detected at an early stage. Utilizing dual RNA-sequencing (RNA-seq), we have simultaneously investigated the gene expression patterns in both, the infecting oomycete ( and infected host (coconut leaflets). Samples were collected at three time points viz., 12, 24 and 36 h, from both infected and uninfected (control) tissues and subjected to RNA-seq on an Illumina Hiseq™ 2500 sequencing platform. High quality reads obtained were subjected to mapping with corresponding reference genomes by using the HISAT2/ StringTie package. A total of 81,683 transcripts were generated against the coconut reference genome, while 9340 transcripts were generated against genome. Out of these, a total of 64,639 coconut transcripts and 9168 transcripts could be annotated using BLASTx. Gene ontology (GO) analysis, carried out using Blast2GO, resulted in 212,643 coconut and 30,736 transcripts being functionally classified, with a single gene product described by numerous terms under the three classifications. The insights obtained could contribute to an understanding of pathogenesis of and inducible defense response of coconut leaves to .

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150523PMC
http://dx.doi.org/10.1016/j.dib.2020.105455DOI Listing

Publication Analysis

Top Keywords

dual rna-sequencing
8
transcripts generated
8
coconut
7
transcripts
5
dataset dual
4
rna-sequencing infecting
4
infecting coconut
4
coconut spp
4
spp oomycetes
4
oomycetes pathogen
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!