Objective: The aim of this work was to investigate three different mutations; Fec-B, FecX, Fec-G at three candidate genes; Bone morphogenetic protein receptor IB, Bone morphogenetic protein 15 and Growth Differentiation Factor 9, respectively, in six sheep breeds reared in Egypt namely; Rahmani, Barki, Rahmani X Barki cross, Awassi, Awassi X Suffolk cross, and Ossimi and their association with litter size.
Results: Genomic DNA of 132 sheep was investigated for the Fec-B, FecX, and Fec-G mutations by Restriction Fragment Length Polymorphism, Single-Stranded Conformation Polymorphism and DNA sequencing. The results revealed that all breeds did not carry Fec-B mutation. On the other side, the mutations of FecX, and Fec-G were detected in Rahmani, and Rahmani X Barki cross which is associated with the high twinning rate/litter size of Rahmani (1.28) and Rahmani X Barki cross (1.22). While, the average litter size for other breeds had almost a constant values rate over six parities, ranging between 1.00 and 1.04.
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http://dx.doi.org/10.1186/s13104-020-05047-9 | DOI Listing |
Animals (Basel)
October 2022
LABCiS, University of Limoges, UR 22722, F-87000 Limoges, France.
(1) Background: It has been recognized that the origin of fat-tailed sheep occurred within coarse wool breeds and that this character was introgressed several times into thin-tailed populations. However, no study has investigated this idea for Egyptian breeds using mtDNA analyses. (2) Methods: Using new sequences of the control region, we constructed a database of 467 sequences representing 37 breeds including fat- and thin-tailed ones with 80 Egyptian individuals belonging to six local breeds (Barki, Fallahi, Ossimi, Rahmani, Saidi, Sohagi).
View Article and Find Full Text PDFArch Anim Breed
August 2022
Department of Wool Technology and Production, Desert Research Center, 1st Mathaf El-Mataryia, 11735, Cairo, Egypt.
The objective of this study was to investigate the genetic polymorphisms in the keratin-associated protein () gene in Barki ( ), Rahmani ( ) and Ossimi ( ) as the three major sheep breeds in Egypt. Subsequently, the detected variants were correlated with important wool traits. The traits included greasy fleece weight (GFW, g), staple length (SL, cm), prickle factor (PF, %), medullated fiber (MF, %), fiber diameter (FD, m), crimp percentage (CR, %) and the standard deviation of FD (SD , m), as well as the subjectively assessed traits of kemp score (KS), handle grade (HG), greasy color grade (GCG), bulk grade (BG), luster grade (LG) and staple structure (SST).
View Article and Find Full Text PDFVet World
February 2021
Department of Cell Biology, National Research Centre, El Buhouth Street, 12311, Dokki, Egypt.
Background And Aim: Sheep productivity in developing countries is crucial, as this animal is an essential source of meat and wool. () plays an important role in the regulation of muscle mass through the regulation of muscle growth, differentiation, and regeneration. The present study sought to investigate genetic variation in the first intron of the gene and the association of variants with growth traits in major sheep breeds in Egypt (Barki, Ossimi, and Rahmani) and Saudi Arabia (Najdi) using polymerase chain reaction (PCR) and sequencing.
View Article and Find Full Text PDFVet World
June 2020
Department of Animal Production, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt.
Aim: The present study was performed to assess the association of single-nucleotide polymorphisms (SNPs) in the fatty acid-binding protein 4 () gene with birth weight (BW), final weight (FW), and average daily gain (ADG) in three Egyptian sheep breeds.
Materials And Methods: Genomic DNA was extracted from the blood samples of 50 male and female individuals representing Ossimi, Rahmani, and Barki sheep breeds. A 407 bp nucleotide (nt) segment from the first intron of was amplified by polymerase chain reaction, sequenced, and analyzed in the different samples.
BMC Res Notes
April 2020
Animal and Fish Production Department, Faculty of Agriculture (Alshatby), Alexandria University, Alexandria, Egypt.
Objective: The aim of this work was to investigate three different mutations; Fec-B, FecX, Fec-G at three candidate genes; Bone morphogenetic protein receptor IB, Bone morphogenetic protein 15 and Growth Differentiation Factor 9, respectively, in six sheep breeds reared in Egypt namely; Rahmani, Barki, Rahmani X Barki cross, Awassi, Awassi X Suffolk cross, and Ossimi and their association with litter size.
Results: Genomic DNA of 132 sheep was investigated for the Fec-B, FecX, and Fec-G mutations by Restriction Fragment Length Polymorphism, Single-Stranded Conformation Polymorphism and DNA sequencing. The results revealed that all breeds did not carry Fec-B mutation.
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