Objectives: This study analyzed salivary samples of COVID-19 patients and compared the results with their clinical and laboratory data.
Methods: Salivary samples of 25 COVID-19 patients were analyzed by rRT-PCR. The following data were collected: age, sex, comorbidities, drugs. Lactate dehydrogenase (LDH) and ultrasensitive reactive C protein (usRCP) values were registered on the same day when a salivary swab was collected. Prevalence of positivity in saliva and association between clinical data and the cycle threshold as a semiquantitative indicator of viral load were considered.
Results: Twenty-five subjects were recruited into this study, 17 males and 8 females. The mean age was 61.5 +/- 11.2 years. Cardiovascular and/or dysmetabolic disorders were observed in 65.22% of cases. All the samples tested positive for the presence of SARS-CoV-2, while there was an inverse association between LDH and Ct values. Two patients showed positive salivary results on the same days when their pharyngeal or respiratory swabs showed conversion.
Conclusions: Saliva is a reliable tool to detect SARS-CoV-2. The role of saliva in COVID-19 diagnosis could not be limited to a qualitative detection of the virus, but it may also provide information about the clinical evolution of the disease.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7194805 | PMC |
| http://dx.doi.org/10.1016/j.jinf.2020.04.005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assesment of Salivary and Serum Levels of HBD2 in Patients with Chronic Angioedema.
J Clin Med
December 2024
Department of Pulmology, Special Hospital for Pulmonary Diseases, 10000 Zagreb, Croatia.
: Human β-defensin 2 (HBD2) is a protein that plays an important role in activating the immune system by modulating spinal pathways and the inflammatory response. According to previous research, HBD2 was proven to be important in chronic spontaneous urticaria (CSU) (their values were significantly elevated in CSU patients, with a significant correlation between HBD2 levels and the percentage of peripheral basophils, suggesting that elevated HBD2 levels may be a potential marker of basophil and mast cell activation), which led us to additional research on the HBD2 molecule in isolated chronic angioedema. The aim of this research is to examine HBD2 values in the saliva and serum of patients with isolated angioedema, as a potential biomarker of the disease.
View Article and Find Full Text PDFEvaluation of Thermal Liquid Biopsy Analysis of Saliva and Blood Plasma Specimens as a Novel Diagnostic Modality in Head and Neck Cancer.
Cancers (Basel)
December 2024
UofL Health-Brown Cancer Center, Department of Medicine, University of Louisville, Louisville, KY 40202, USA.
Background: Over the past decade, saliva-based liquid biopsies have emerged as promising tools for the early diagnosis, prognosis, and monitoring of cancer, particularly in high-risk populations. However, challenges persist because of low concentrations and variable modifications of biomarkers linked to tumor development when compared to normal salivary components.
Methods: This study explores the application of differential scanning calorimetry (DSC)-based thermal liquid biopsy (TLB) for analyzing saliva and blood plasma samples from head and neck cancer (HNC) patients.
Entropy-driven "two-way signal output" cyclic circuit: An ultra-sensitive electrochemical biosensor for non-invasive ORAOV 1 detection.
Talanta
January 2025
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, China. Electronic address:
The rapid, sensitive and reliable detection of oral cancer overexpressed 1 (ORAOV 1) is crucial for the early, non-invasive diagnosis of oral squamous cell carcinoma (OSCC). Herein, we are the first to construct an ultrasensitive electrochemical (EC) biosensor based on an entropy-driven "two-way signal output" (TWSO) cyclic circuit for salivary ORAOV 1 detection. This innovative TWSO cyclic circuit can skillfully convert by-products into desired signal-generating units, not only reducing the excessive accumulation of by-products but also improving the utilization efficiency of output chains, thereby achieving rapid reaction kinetics and high signal outputs.
View Article and Find Full Text PDFLab-on-the-Needles: A Microneedle Patch-Based Mobile Unit for Highly Sensitive and Detection of Protein Biomarkers.
ACS Nano
January 2025
Department of Biomedical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
Detection of biomarkers associated with physiological conditions provides critical insights into healthcare and disease management. However, challenges in sampling and analysis complicate the detection and quantification of protein biomarkers within the epidermal layer of the skin and in viscous liquid biopsy samples. Here, we present the "Lab-on-the-Needles" concept, utilizing a microneedle patch-based sensing box (MNP-based SenBox) for mobile healthcare applications.
View Article and Find Full Text PDFA novel LC-MS/MS assay for low concentrations of creatinine in sweat and saliva to validate biosensors for continuous monitoring of renal function.
J Chromatogr B Analyt Technol Biomed Life Sci
December 2024
Clinical Laboratory, Catharina Hospital Eindhoven, Eindhoven 5623 EJ, The Netherlands; Department of Biomedical Engineering, Chemical Biology, Eindhoven University of Technology, Groene Loper 3, Eindhoven 5612 AE, The Netherlands.
Monitoring of kidney function traditionally relies on plasma creatinine concentrations, necessitating invasive blood draws. Non-invasively obtainable biofluids, such as sweat and saliva, present a patient-friendly alternative with potential for continuous monitoring. This study focusses on developing and validating a novel Liquid Chromatography- tandem Mass Spectrometry (LC-MS/MS) assay as a reference test for measuring low creatinine concentrations in sweat and saliva.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!