AI Article Synopsis
- - The study focuses on Runting-Stunting Syndrome (RSS) in chickens, a disease causing significant economic losses due to malabsorption, which has unclear causes despite decades of research.
- - Researchers analyzed viral metagenomes from four affected chicken flocks using next-generation sequencing and developed specific primers to identify various enteric viruses linked to RSS.
- - Findings revealed a strong association between chicken astrovirus and RSS, with this virus detected in all samples; additionally, two previously unreported viruses were discovered, contributing to a better understanding of viral presence in chicken enteric diseases.
Article Abstract
Background And Aims: Runting-stunting syndrome (RSS) in chickens, also known as malabsorption syndrome, which is characterized by mild to severe enteritis and diagnosed through typical histopathologic examination as well as clinical signs, results in considerable economic losses. Despite the many studies carried out over decades to determine the etiologic agents of RSS involved in the disease, several outbreaks remained without the elucidation of, potentially multiple, etiologies involved.
Methods: We performed comparative analysis of viral metagenomes from four chicken flocks affected with RSS using next-generation sequencing. Primers for the detection of chicken enteric viruses were designed from the sequencing data obtained with metagenomics. Multiplex reverse transcription-polymerase chain reaction (PCR) and PCR were performed to detect a variety of etiological agents previously described in natural cases of RSS.
Results: The most abundant viral families identified in this study were Astroviridae, Picornaviridae, Parvoviridae, Caliciviridae, Reoviridae and Picobirnaviridae. Chicken astrovirus sequences were present in all four samples, suggesting an association between chicken astrovirus and RSS and chicken astrovirus as a candidate pathogen responsible for RSS. Picobirnavirus and the newly identified chapparvovirus were found in chickens in the Republic of Korea for the first time, and the genetic diversity of enteric viruses and viral communities was showed.
Conclusions: Chicken astrovirus was consistently detected in broilers affected with RSS and the result of this study may contribute to knowledge of enteric diseases and viruses in chickens.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7157833 | PMC |
| http://dx.doi.org/10.1186/s12985-020-01307-z | DOI Listing |
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