Development and testing of a novel killer-rescue self-limiting gene drive system in .

Proc Biol Sci

Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27615, USA.

Published: April 2020

Here we report the development and testing of a novel self-limiting gene drive system, Killer-Rescue (K-R), in . This system is composed of an autoregulated Gal4 Killer (K) and a Gal4-activated Gal80 Rescue (R). Overexpression of Gal4 is lethal, but in the presence of R activation of Gal80 leads to much lower levels of Gal4 and rescue of lethality. We demonstrate that with a single 2 : 1 engineered to wild-type release, K drives R through the population and after nine generations, more than 98% of the population carry R and less than 2% of the population are wild-type flies. We discuss how this simple K-R gene drive system may be readily adapted for population replacement in a human health pest, , or for population suppression in an agricultural pest, .

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211443PMC
http://dx.doi.org/10.1098/rspb.2019.2994DOI Listing

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