AI Article Synopsis
- - This study investigates how various factors like flow rate, velocity, DNA concentration, channel height, and time affect the process of DNA hybridization in microfluidics.
- - Using a syringe pump, the researchers sent Cy3-labeled target DNA through a PDMS microfluidic channel to bind with DNA from the West Nile Virus.
- - They found that lowering the channel height improves the delivery of target DNA to capture probes, leading to higher fluorescence for lower DNA concentrations, with optimal results achieved at a high flow rate and low channel height in just 2 minutes.
Article Abstract
In this investigation we report on the influence of volumetric flow rate, flow velocity, complementary DNA concentration, height of a microfluidic flow channel and time on DNA hybridization kinetics. A syringe pump was used to drive Cy3-labeled target DNA through a polydimethylsiloxane (PDMS) microfluidic flow channel to hybridize with immobilized DNA from the West Nile Virus. We demonstrate that a reduction of channel height, while keeping a fixed volumetric flow rate or a fixed flow velocity, enhances mass transport of target DNA to the capture probes. Compared to a passive hybridization, the DNA hybridization in the microfluidic flow channel generates higher fluorescence intensities for lower concentration of target DNA during the same fixed period of time. Within a fixed 2 min time period the fastest DNA hybridization at a 50 pM concentration of target DNA is achieved with a continuous flow of target DNA at the highest flow rate and the lowest channel height.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125766 | PMC |
| http://dx.doi.org/10.1016/j.snb.2005.03.034 | DOI Listing |
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