AI Article Synopsis
- The study explores how low intensity ultrasound (LIUS) affects cell adhesion at two levels: basal (cell-substrate) and apical (cell-cell) in mouse mammary gland epithelial cells (EpH4).
- It focuses on the phosphorylation of a protein called p130CAS, which serves as a marker for cellular responses to ultrasound stimulation.
- Results indicate that ultrasound induces immediate and significant phosphorylation of p130CAS, suggesting a dose-dependent mechanosensitive response for both cell-substrate and cell-cell adhesion, with a specific link to E-cadherin in modified cell models.
Article Abstract
In this study, we investigated the cellular mechanosensitive responses to a low intensity ultrasound (LIUS) stimulation (ISATA = 1 mW/cm2, pressure = 10 kPa). The dose and temporal effects at cell-substrate adhesion (CSA) at the basal level and cell-cell adhesion (CCA) at the apical level are reported in detail. A model of mouse mammary gland epithelial cells (EpH4) and the phosphorylation of mechanosensitive 130 kDa Crk-associated substrate (p130CAS) as an indicator for cellular responses were used. The intensity of phospho-p130CAS was found to be dependent on LIUS stress level, and the p130CAS was phosphorylated after 1 min stimulation at CSA. The phospho-p130CAS was also found to increase significantly at CCA upon LIUS stimulation. We confirmed that the cellular responses to ultrasound are immediate and dose dependent. Ultrasound affects not only CSA but also CCA. An E-cadherin knockout (EpH4ECad-/-) model also confirmed that phosphorylation of p130CAS at CCA is related to E-cadherins.
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| http://dx.doi.org/10.1115/1.4046883 | DOI Listing |
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