AI Article Synopsis
- Transgenic technologies are key tools in biotechnology, allowing for targeted genetic modifications that can uncover gene functions and alter plant metabolic pathways.
- Proper design of chimeric gene constructs is crucial for effective modification, particularly in the choice of promoters that regulate transgene expression.
- The TGP database has been created to gather information on various noncanonical tissue-specific and developmentally regulated promoters, providing resources for optimizing transgene control in plants.
Article Abstract
Transgenic technologies belong to important tools of reverse genetics and biotechnology in plants. Targeted genetic modifications can reveal functions of genes of interest, change metabolic and regulatory pathways, or result in accumulation of valuable proteins or metabolites. However, to be efficient in targeted genetic modification, the chimeric gene construct should be designed properly. In particular, the promoters used to control transgene expression need to be carefully chosen. Most promoters in widely used vectors belong to strong and constitutively expressed variants. However, in many cases transgene expression has to be restricted to certain tissue, stage of development, or response to some internal or external stimuli. In turn, a large variety of tissue-specific promoters have been studied and information on their characteristics may be recovered from the literature. An appropriate promoter may be selected and used in genetic construct to optimize the transgene transcription pattern. We have previously designed the TGP database (TransGene Promoters, http://wwwmgs.bionet.nsc.ru/mgs/dbases/tgp/home.html ) collecting information from the publications in this field. Here we review the wide range of noncanonical tissue-specific and developmentally regulated promoters that might be used for transgene expression control.
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| http://dx.doi.org/10.1007/978-1-0716-0356-7_4 | DOI Listing |
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