AI Article Synopsis
- Fungal metabolism varies based on its growth stage and the conditions it’s cultivated in.
- Solid culture methods mimic natural conditions better than liquid culture but have limitations in harvesting and modifying the medium.
- The new float culture method allows for colony development akin to solid culture while facilitating the production of high-quality RNA samples.
Article Abstract
Fungal metabolism is affected by both the developmental stage and cultivation conditions. Fungal growth in solid culture reflects natural conditions more closely than growth in liquid culture; however, because the mycelium cannot be harvested easily and the medium composition cannot be modified during incubation, the approach has some limitations when compared to liquid culture methods. The float culture incubation method introduced herein enables fungus to develop similar colonies to those on solid culture. This is a simple method that leads to the production of high-quality RNA samples.
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| http://dx.doi.org/10.1016/j.ab.2020.113722 | DOI Listing |
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