AI Article Synopsis
- Calcineurin, a protein phosphatase activated by increased intracellular calcium levels, plays a crucial role in cellular processes across various organisms, from yeast to humans.
- It helps fungi adapt to environmental challenges and is vital for the virulence of pathogenic species.
- Researchers have developed GFP transcriptional reporters to track calcineurin activity in live yeast cells, allowing for real-time monitoring of its dynamics during stress and aging, and facilitating drug screening for potential antifungal treatments.
Article Abstract
The protein phosphatase calcineurin is activated in response to rising intracellular Ca levels and impacts fundamental cellular processes in organisms ranging from yeast to humans. In fungi, calcineurin orchestrates cellular adaptation to diverse environmental challenges and is essential for virulence of pathogenic species. To enable rapid and large-scale assessment of calcineurin activity in living, unperturbed yeast cells, we have generated stable and destabilized GFP transcriptional reporters under the control of a calcineurin-dependent response element (CDRE). Using the reporters, we show that the rapid dynamics of calcineurin activation and deactivation can be followed by flow cytometry and fluorescence microscopy. This system is compatible with live/dead staining that excludes confounding dead cells from the analysis. The reporters provide technology to monitor calcineurin dynamics during stress and ageing and may serve as a drug-screening platform to identify novel antifungal compounds that selectively target calcineurin.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136757 | PMC |
| http://dx.doi.org/10.15698/mic2020.04.713 | DOI Listing |
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