A dual-functional probe for sensing pH change and ratiometric detection of Cu.

A series of benzothiazole-based compounds were synthesized and characterized. Among them, probe Z showed significant dual-functional performance which was capable of sensing pH change and Cu. Probe Z displayed fluorescent turn-on under alkaline conditions due to deprotonation of the hydroxyl group along with the obviously color change from colorless to mint green. Interestingly, it further achieved in ratiometric detection of Cu through absorbance or fluorescence signals in strong alkaline condition. The limit of detection was calculated correspondingly as 0.37 μM and 1.35 μM, respectively. Especially, the combination of the XNOR and INHIBIT logic gates could be used to confirm that one medium was in neutrality or alkalinity condition. Moreover, Z was successfully used in real water samples and test paper for fast identification of Cu, respectively.