PNAd and MAdCAM-1 addressins on venules are of importance in T-cell homing and potential therapeutic targets in ulcerative colitis (UC). Normally, PNAd high endothelial venules (HEVs) are only present in lymphoid organs, whereas small numbers of MAdCAM-1 venules can be seen in non-lymphoid tissue. We aimed to study their presence in the intestinal mucosa of UC patients at diagnosis and during follow-up, and their correlation with disease activity. Colonic biopsy specimens of 378 UC patients were analyzed by immunohistochemistry for CD3, CD20, ERG, MECA-79 (PNAd) and MECA-376 (MAdCAM-1) and compared to healthy controls (HC). The proportion of PNAdHEVs in UC at diagnosis was 4.9% (IQR 2.0%-8.3%), while none were detected in HC. During follow-up, PNAdHEVs completely disappeared in remission ( = 93), whereas the proportion in active disease was similar to baseline ( = 285, = 0.39). The proportion of MAdCAM-1venules in UC at baseline was 5.8% (IQR 2.6-10.0). During follow-up, the proportion in remission was comparable to diagnosis, but upregulated (7.5% (IQR 4.4-10.9), = 0.001) in active disease. In conclusion, PNAdHEVs appear in UC during active inflammation which could thus serve as a marker for disease activity, whereas MAdCAM-1venules remain present after inflammation is resolved and increase after subsequent flares, reflecting chronicity and potentially serving as a therapeutic target.
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