To investigate immune activity of pine nut peptides treated by PEF technology and mechanism of targeting immunoactive sitesits, immune regulatory active was evaluated by RAW 264.7 cells model and the structures of pine nut peptides were researched by fluorescence, CD, and 1D/2D NMR spectrum. These consequences showed the ability of macrophages to phagocytosis neutral red and the production of nitric oxide (NO) were improved after PEF treatment. KWFCT treated by PEF treatment with 40 kV/cm obtained the best immunocompetence. The CD spectroscopy showed that PEF could transform the secondary structures of pine nut peptides. The short-range correlation between C H (1.65 ppm) and C H (3.35 ppm), and long-range correlation between C H (3.37 ppm) and N H (8.07 ppm) were enhanced by PEF treatment. PEF treatment of tryptophan in the pine nut peptides enhanced the immunological activity of the pine nut peptides. PRACTICAL APPLICATIONS: Bioactive peptides derived from food proteins have been extensively studied in recent years. However, little research has been done on the immunoactive peptide of pine nut source. PEF treatment is promising for improving certain properties of foods while maintaining the flavor, color, taste, and nutritional value of the food. This research demonstrated that PEF treatment increased the immunological activity of KWFCT and KWFM. The primary structure of KWFCT and KWFM did not change after PEF treatment, but the secondary structure was transformed into each other. A new perspective on the PEF action site is proposed, which is beneficial to the application of PEF technology.
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http://dx.doi.org/10.1111/jfbc.13224 | DOI Listing |
J Nutr
January 2025
Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, 03760, Republic of Korea; Graduate Program in System Health Science and Engineering, Ewha Womans University, Seoul, 03760, Republic of Korea. Electronic address:
Background: Pine (Pinus koraiensis) nut oil (PNO) has been reported to have various beneficial effects on hepatic triglyceride accumulation and atherosclerosis in animal models. MicroRNAs (miRs) are involved in various diseases by modulating physiological processes. However, the mechanism underlying PNO's effects on the regulation of miRs involved in hepatic cholesterol homeostasis and inflammation remains unclear.
View Article and Find Full Text PDFHeliyon
July 2024
Plekhanov Russian University of Economics, Faculty of Trade Economics and Commodity Science, Department of Commodity Science, Stremyanny lane 36, RU 115054, Moscow, Russia.
The impact of thermal treatment for roasting on pine nuts from two geographical regions in Russia (Vladivostok and Baikal) was studied. They were roasted at 180 °C for 20 min in an oven. The aim was to establish the changes that occurred in the chemical (proteins, lipids, carbohydrates, ash, fibers), lipid composition (fatty acids, tocopherols, sterols, phospholipids), and physicochemical characteristics (peroxide value, acid value, iodine value, conjugated dienes and trienes) after roasting of the pine nuts.
View Article and Find Full Text PDFFood Sci Nutr
January 2025
Caspian Sea Ecology Research Center Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization Mazandaran Iran.
Rainbow trout () is a freshwater fish susceptible to chemical and microbial spoilage, limiting its shelf life. This study aimed to enhance and extend the rainbow trout fillets' shelf life stored at 4°C ± 1°C through an immersion treatment using ultrasound-assisted, defatted pine nut ( Wallich) extracts at concentrations of 1% and 2% (w/v), compared to the control group (0% pine nut). Evaluations were conducted at storage intervals of 0, 4, 8, 12, 16, and 20 days.
View Article and Find Full Text PDFJ Fluoresc
December 2024
Jiamusi University, Jiamusi, 154007, China.
In this work, Waste pine nut shells were used as organic carbon source of biomass to synthesize carbon quantum dots. A highly responsive and selective fluorescent nanosensor (Si-doped biomass-derived carbon dots with molecular imprinted polymers, Si-CDs@MIPs) was designed for determination of Rutin (RT) in Chinese herbal substances like Sophora japonica L..
View Article and Find Full Text PDFFood Chem
March 2025
Department of Biochemistry, Memorial University of Newfoundland, St. John 's, NL, Canada.
This article presents a comprehensive overview of upcycling commercial nut byproducts (such as Brazil nut, cashew, hazelnut, macadamia, peanut (also known as a legume), pecan, pine nut, pistachio, and walnut) for food, nutraceutical, and pharmaceutical applications. Upcycling nut byproducts, namely husk/hull, hard shell, brown skin, defatted flour/meal/cake, pine cone, cashew nut shell liquid, cashew apple, walnut septum, and dreg/okara, has great potential, not only to reduce/minimise waste, but also to fit within the circular economy concept. Each byproduct has its own unique functional properties, which can bring significant value.
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