AI Article Synopsis
- Mammary gland development consists of three stages: puberty, pregnancy and lactation, and involution, involving significant tissue remodeling.
- Three-dimensional (3D) mammary organoid culture is a vital tool for studying mammary gland biology and has advanced our understanding of pubertal development and breast cancer.
- The study presents a new mouse mammary organoid model that effectively mimics lactation and involution processes, providing a valuable platform for researching physiological mechanisms and pregnancy-related breast cancer.
Article Abstract
Mammary gland development occurs mainly after birth and is composed of three successive stages: puberty, pregnancy and lactation, and involution. These developmental stages are associated with major tissue remodeling, including extensive changes in mammary epithelium, as well as surrounding stroma. Three-dimensional (3D) mammary organoid culture has become an important tool in mammary gland biology and enabled invaluable discoveries on pubertal mammary branching morphogenesis and breast cancer. However, a suitable 3D organoid model recapitulating key aspects of lactation and involution has been missing. Here, we describe a robust and straightforward mouse mammary organoid system modeling lactation and involution-like process, which can be applied to study mechanisms of physiological mammary gland lactation and involution as well as pregnancy-associated breast cancer.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098375 | PMC |
| http://dx.doi.org/10.3389/fcell.2020.00068 | DOI Listing |
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Article Synopsis
- The mammary gland experiences significant structural and compositional changes throughout a woman's life, mainly influenced by hormones and environmental factors, including phases like pregnancy and menopause.
- Research using rodent models has been helpful in understanding these processes but doesn't completely represent human development, leading to interest in using rabbits as alternative models due to their similarities to human mammary biology.
- A new methodology has been developed for preparing and analyzing rabbit mammary gland whole mounts, addressing the technical challenges of thicker tissue and paving the way for better research on mammary gland growth and the impacts of hormonal or toxicant disruptions.
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