Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude extract systems, as well as breakthroughs in enabling technologies, that have facilitated increased throughput, compartmentalization, and spatial control of cell-free protein synthesis reactions. Combined with a deeper understanding of the cell-free systems themselves, these advances improve our ability to address a range of scientific questions. By mastering control of the cell-free platform, we will be in a position to construct increasingly complex biomolecular systems, and approach natural biological complexity in a bottom-up manner.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105575 | PMC |
| http://dx.doi.org/10.3389/fbioe.2020.00213 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST.
Sci Adv
January 2025
Department of Cell Biology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.
Lysosomal storage diseases (LSDs) comprise ~50 monogenic disorders marked by the buildup of cellular material in lysosomes, yet systematic global molecular phenotyping of proteins and lipids is lacking. We present a nanoflow-based multiomic single-shot technology (nMOST) workflow that quantifies HeLa cell proteomes and lipidomes from over two dozen LSD mutants. Global cross-correlation analysis between lipids and proteins identified autophagy defects, notably the accumulation of ferritinophagy substrates and receptors, especially in and mutants, where lysosomes accumulate cholesterol.
View Article and Find Full Text PDFMultiorifice acoustic microrobot for boundary-free multimodal 3D swimming.
Proc Natl Acad Sci U S A
January 2025
Physical Intelligence Department, Max Planck Institute for Intelligent Systems, Stuttgart 70569, Germany.
The emerging new generation of small-scaled acoustic microrobots is poised to expedite the adoption of microrobotics in biomedical research. Recent designs of these microrobots have enabled intricate bioinspired motions, paving the way for their real-world applications. We present a multiorifice design of air-filled spherical microrobots that convert acoustic wave energy to efficient propulsion through a resonant encapsulated microbubble.
View Article and Find Full Text PDFFrom human to superhuman: the impact of the microbiome on physiology.
J Physiol
January 2025
Department of Physiology and Membrane Biology, University of California Davis School of Medicine, Sacramento, CA, USA.
The complex microbial community residing in the human gut has long been understood to regulate gastrointestinal physiology and to participate in digestive diseases, but its extraintestinal actions and influences are increasingly recognized. This article discusses bidirectional interactions between the gut microbiome and athletic performance, metabolism, longevity and the ability of the gut-brain axis to influence cognitive function and mental health.
View Article and Find Full Text PDFUniversal Hydrogel Carrier Enhances Bone Graft Success: Preclinical and Clinical Evaluation.
Adv Healthc Mater
January 2025
School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, NSW, 2006, Australia.
Orthopedic, maxillofacial, and complex dentoalveolar bone grafting procedures that require donor-site bone harvesting can be associated with post-surgical complications. There has been widespread adoption of exogenously sourced particulate bone graft materials (BGM) for bone regenerative procedures; however, the particulate nature of these materials may lead to compromised healing outcomes, mainly attributed to structural collapse of the BGM, prolonged tissue healing. In this study, a fully synthetic thermoresponsive hydrogel-based universal carrier matrix (TX) that forms flowable and shapable putties with different BGMs while spatially preserving the particles in a 3D scaffold at the implantation site is introduced.
View Article and Find Full Text PDFReactions of Surface Peroxides Contribute to Rates and Selectivities for CH Epoxidation on Silver.
ACS Catal
January 2025
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.
Partial oxidation of ethylene over silver catalysts produces more than 30 million metric tons of ethylene oxide (EO) annually. However, the form of active silver surfaces, reactive oxygen species, and dominant pathways of this chemical reaction remains controversial despite decades of research. Here, we use Raman spectroscopy and transient kinetic measurements to demonstrate that higher coverages of peroxide species, present only upon Ag oxide surfaces that form , correlate with greater selectivities to EO.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!