In this work, red-emitting carbon dots (R-CDs) with a high quantum yield (QY) of 28% in water were synthesized for the first time by heating an ethanol solution of pulp-free lemon juice. The obtained R-CDs were mono-dispersed with an average diameter of 4.6 nm, and exhibited excitation-independent emission at 631 nm. Meanwhile, these R-CDs featured low cytotoxicity and good photostability, which allow R-CDs to be employed as luminescent probes for in vitro/in vivo bioimaging. In addition, a detailed study on the physical properties and structural compositions of the sodium borohydride (NaBH) reduced R-CDs with orange emission suggested that surface states on the R-CD surfaces and nitrogen-derived structures in the R-CD cores synergistically caused their intense red luminescence. The low-cost and eco-friendly synthesis method and favorable optical properties of R-CDs make these carbon dots promising for further applications, such as bioimaging and light-emitting diodes.
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