In this paper we for the first time report a reversible sol-gel-sol approach to obtain optical enzymatic biosensors with improved enzyme stability and good sensitivity by using desktop inkjet printing. The developed technique is based on the bio-inorganic inks allowing for a sol-gel-sol transition of the inorganic matrix: from liquid ink to a solid alumina matrix with entrapped enzymes and a subsequent color response due to the enzymatic reaction upon the resuspension of the matrix. This approach improves the stability of the enzymes entrapped in the porous inorganic matrix, and at the same time maintains a high sensitivity of the biomolecules, whose facile release is ensured by the gel-sol transition. Rheological parameters of the developed bio-inorganic ink are highly adjustable making it suitable for the deposition on different surfaces by inkjet printing. The potential and utility of this approach is demonstrated by a successful production of optical biosensors for glucose and uric acid.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c6tb02559e | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An injectable thermosensitive hydrogel with a self-assembled peptide coupled with an antimicrobial peptide for enhanced wound healing.
J Mater Chem B
August 2022
College of Animal Science, Jilin University, Changchun, 130012, China.
A wound dressing based on a thermosensitive hydrogel shows advantages over performed traditional dressings, such as rapid reversible sol-gel-sol transition properties and the capacity to fill an irregular-shaped wound area. Herein, RA-Amps was fabricated by coupling a self-assembled peptide RADA16 with an antibacterial peptide (Amps) and incorporated into a PNIPAM hydrogel containing an MGF E peptide to develop a multi-functional composite hydrogel with thermo-response properties, good biocompatibility, good mechanical properties, and antibacterial and carrier functions for wound healing. PNI/RA-Amps is an injectable thermo-reversible system with a phase transition temperature of ∼32 °C, and exhibits a rapid reversible sol-gel-sol transition of ∼23 s, which makes it conducive to sealing the wound area and avoiding sol diffusion caused by a lengthy gel time.
View Article and Find Full Text PDFDilution-induced gel-sol-gel-sol transitions by competitive supramolecular pathways in water.
Science
July 2022
Institute for Complex Molecular Systems, Laboratory of Macromolecular and Organic Chemistry, Eindhoven University of Technology, 5600 MB Eindhoven, Netherlands.
Fascinating properties are displayed by synthetic multicomponent supramolecular systems that comprise a manifold of competitive interactions, thereby mimicking natural processes. We present the integration of two reentrant phase transitions based on an unexpected dilution-induced assembly process using supramolecular polymers and surfactants. The co-assembly of the water-soluble benzene-1,3,5-tricarboxamide (BTA-EG) and a surfactant at a specific ratio yielded small-sized aggregates.
View Article and Find Full Text PDFThermoresponsive BSA hydrogels with phase tunability.
Mater Sci Eng C Mater Biol Appl
February 2021
Dept. of Chemical Engineering, Indian Institute of Technology Delhi, New Delhi 110016, India. Electronic address:
Amyloids are fibrillar structures formed due to protein aggregation or misfolding when the molecules undergo a conformational change from α-helix to β-sheet. Although this self-assembly is associated with many neurodegenerative diseases in vivo, the highly ordered amyloidic structures formed in vitro are ideal scaffolds for many bionanotechnological applications. Amyloid fibrillar networks under specific stimuli can also form stable hydrogels.
View Article and Find Full Text PDFReversible sol-gel-sol medium for enzymatic optical biosensors.
J Mater Chem B
January 2017
Laboratory of Solution Chemistry of Advanced Materials and Technologies, ITMO University, St. Petersburg 197101, Russian Federation.
In this paper we for the first time report a reversible sol-gel-sol approach to obtain optical enzymatic biosensors with improved enzyme stability and good sensitivity by using desktop inkjet printing. The developed technique is based on the bio-inorganic inks allowing for a sol-gel-sol transition of the inorganic matrix: from liquid ink to a solid alumina matrix with entrapped enzymes and a subsequent color response due to the enzymatic reaction upon the resuspension of the matrix. This approach improves the stability of the enzymes entrapped in the porous inorganic matrix, and at the same time maintains a high sensitivity of the biomolecules, whose facile release is ensured by the gel-sol transition.
View Article and Find Full Text PDFControlled release bevacizumab in thermoresponsive hydrogel found to inhibit angiogenesis.
Biomed Mater Eng
June 2015
Department of Life Science, Fu Jen Catholic University, New Taipei City, Taiwan.
Age-related macular degeneration (ARMD) and intraocular neovascular diseases have been treated clinically by anti-VEGF antibody drug bevacizumab. However, the use of bevacizumab in the treatment of retinal neovascular diseases has been limited due to the short half-life and frequent injections. In this research, novel amphiphilic hydrophilic-hydrophobic block copolymers of methoxy-poly (ethylene glycol)-block-poly (lactic-co-glycolic acid) were synthesized with ring-opening polymerization, and cross-linked with 2,2-bis (2-oxazoline) (BOX).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!