We integrated for the first time a novel dye-intercalated DNA dendrimer probe (GSG) with the electrospun nanofibers (NFs) to create an amplified fluorescent sensing platform for the detection of nucleic acids, proteins, and cancer cells. The sensing platform is constructed based on the formation of the NF-capture/target/reporter-GSG sandwich-type nanocomplex on the NF membrane surface, leading to large fluorescence signal amplification. Taking advantage of the dual-amplification of both the strong emission intensity of GSG and the large surface area-to-volume ratio of NFs, this platform enables highly sensitive detection of 20 pM of a β-thalassemia gene fragment. Furthermore, by easily altering the correlative capture and reporter sequences, the proposed sensing platform can also be applied to detect various targets including thrombin and HeLa cells with high sensitivity and selectivity. This work would provide a simple and versatile strategy for detecting disease-related species in biological samples.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c5tb00357a | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Experiences of women of childbearing age with diabetes skin pruritus: An interpretative phenomenological analysis.
Diabetes Res Clin Pract
January 2025
Nursing Department, Hunan Provincial Hospital of Integrated Traditional Chinese and Western Medicine (the Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine), No. 58, Lushan Road, Changsha City, Hunan Province 410006, China. Electronic address:
Aim: This study aims to explore the experiences and feelings of women of childbearing age with diabetes skin pruritus after being diagnosed.
Methods: Using the Interpretative Phenomenological Analysis method, semi-structured interviews were conducted with women of childbearing age who suffer from diabetes skin pruritus, followed by data analysis.
Results: Three superordinate themes and eight subthemes were identified, including psychological distress (self-identity, lack of understanding from others, emotional damage and self-disgust), physical impairment (loss of bodily control, treatment challenges, impact on female sexual characteristics), and coping post-trauma (social withdrawal, active coping).
TiCT/Au NPs/PPy ternary heterostructure-based intra-capacitive self-powered sensor for DEHP detection.
J Hazard Mater
January 2025
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China. Electronic address:
Phthalate esters, particularly di(2-ethylhexyl) phthalate (DEHP), are widely used plasticizers found in various consumer products, posing significant environmental and health risks due to their endocrine-disrupting effects. In this study, a novel enzyme-free intra-capacitive biofuel cell self-powered sensor (ICBFC-SPS) was developed. The ICBFC-SPS integrated a ternary heterostructure-based capacitive anode and a cathode with a sensing interface into a single-chamber electrolytic cell.
View Article and Find Full Text PDFMonitoring phycocyanin in global inland waters by remote sensing: Progress and future developments.
Water Res
January 2025
State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.
Cyanobacterial blooms are increasingly becoming major threats to global inland aquatic ecosystems. Phycocyanin (PC), a pigment unique to cyanobacteria, can provide important reference for the study of cyanobacterial blooms warning. New satellite technology and cloud computing platforms have greatly improved research on PC, with the average number of studies examining it having increased from 5 per year before 2018 to 17 per year thereafter.
View Article and Find Full Text PDFA chitosan-based sensing membrane for on-site and sensitive dual-channel portable detection and efficient adsorption of Pb in groundwater.
Anal Chim Acta
February 2025
State Key Laboratory of Applied Organic Chemistry and Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Gansu, Lanzhou, 730000, China. Electronic address:
The presence of lead ion (Pb) in groundwater poses a serious risk to human health, even at low levels. Therefore, it is essential to develop a new strategy for both selective detection and effective removal of Pb in groundwater, which has been rarely reported. Here, we developed a multi-functional chitosan-based fluorescent sensing membrane (CM-L/CG) by using a casting method for the sensitive/selective detection and removal of Pb in groundwater.
View Article and Find Full Text PDFBiological studies reveal the role of trpA gene in biofilm formation, motility, hemolysis and virulence in Vibrio anguillarum.
Microb Pathog
January 2025
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Marine Biomedical Science and Technology Innovation Platform of Lin-gang Special Area, Shanghai 201306, China. Electronic address:
Vibrio anguillarum is a pathogen responsible for vibriosis in aquaculture animals. The formation of bacterial biofilm contributes to infections and increases resistance to antibiotics. Tryptophanase and its substrate tryptophan have been recognized as signal molecules regulating bacterial biofilm formation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!