A novel ratiometric fluorescent chemodosimeter has been developed for reusable detection of Hg. The chemodosimeter responds to Hg sensitively and selectively with a remarkable fluorescence change from green to blue through hampering the excited state intramolecular proton transfer (ESIPT) process. This recyclable chemodosimeter can remove Hg from water by forming a unique mercury-containing compound, which could be reused in the presence of NaBH. Moreover, the chemodosimeter exhibits a ratiometric fluorescence response to Hg with a very low detection limit (1.0 ppb), and it can be used to detect Hg in drinking water. Furthermore, the ratiometric chemodosimeter has been successfully used for imaging Hg in living cells and tissues using two-photon fluorescence microscopy due to the remarkable emission change from green to blue. This provides a novel testing method for detecting Hg in living cells and tissues with low cytotoxicity and autofluorescence.
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