Covalent immobilization of ssDNA fragments onto silicon-based materials was performed using the thiol-yne reaction. Chemical functionalization provided alkyne groups on the surface where the thiol-modified oligonucleotide probes can be easily photoattached as microarrays, reaching an immobilization density around 30 pmol cm. The developed method presents the advantages of spatially controlled probe anchoring (by using a photomask), direct attachment without using cross-linkers, and short irradiation times (20 min). Hybridization efficiencies up to 70%, with full complementary strands, were reached. The approach was evaluated by scoring single nucleotide polymorphisms with a discrimination ratio around 15. Moreover, the potential applicability of the proposed methodology is demonstrated through the specific detection of 20 nM of a genomic target of bacterial Escherichia coli.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c4tb01108b | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Triphenyl-Modified Mixed-Mode Stationary Phases With and Without Embedded Ion-Exchange Sites for High-Performance Liquid Chromatography.
J Sep Sci
December 2024
Institute of Pharmaceutical Sciences, Pharmaceutical (Bio-)Analysis, University of Tübingen, Tübingen, Germany.
The present work reports on the preparation, characterization, and evaluation of a set of novel triphenyl-modified silica-based stationary phases without and with embedded ion-exchange sites for mixed-mode liquid chromatography. The three synthesized triphenyl phases differed in additionally incorporated ion-exchange sites. In one embodiment, allyltriphenylsilane was bonded to thiol-modified silica by thiol-ene click reaction, leading to particles with no ion-exchange sites.
View Article and Find Full Text PDFClick Chemistry for Well-Defined Graft Copolymers.
Polymers (Basel)
November 2024
Department of Applied Chemistry, Chemical Engineering, and Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa 992-8510, Yamagata, Japan.
Graft copolymers have gained significant importance in various fields due to their tunable functionality and well-defined architecture. However, there are still limitations due to the compatibility of monomers and functional groups depending on the polymerization mode. Click chemistry has solved this problem through its ability to easily and quantitatively link a wide range of polymers and functional groups.
View Article and Find Full Text PDFDesigning Phenolphthalein-Based Adsorptive Membranes for the High-Affinity, High-Capacity Capture of Contaminants from Water.
ACS Appl Mater Interfaces
December 2024
Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556, United States.
The selective removal of solutes is crucial for ensuring a sustainable water supply, recovering resources, and cost-effective biomanufacturing. Adsorptive membranes are promising in this regard due to their rapid mass transfer and low energy demands. However, state-of-the-art adsorptive membranes offer limited pore sizes and surface chemistries.
View Article and Find Full Text PDF"Click" post-synthesis of chiral microporous organic network for chiral high-performance liquid chromatographic separation.
Anal Chim Acta
December 2024
School of Pharmaceutical Sciences & Institute of Materia Medica, Medical Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong 250117, China. Electronic address:
Article Synopsis
- Chiral separation is crucial in analytical chemistry, and while high-performance liquid chromatography (HPLC) is an effective method, the use of chiral microporous organic networks (MONs) in this context has not been explored previously.
- The study introduces MON@SiO-L-Cys, a newly developed chiral stationary phase that shows promising results in separating various chiral compounds with high resolution and selectivity, outperforming some commercial chiral columns.
- This research highlights the potential of chiral MONs in HPLC applications and suggests innovative approaches for future chiral separations using these materials.
Synthesis of Clickable Isotactic Poly(vinyl ether)s through Organocatalytic Stereoselective Cationic Copolymerization.
Chemistry
November 2024
State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
By virtue of the high reliability of click chemistry, polymers with clickable groups provide a useful platform for the rapid synthesis of polymer materials with diverse functionalities and architectures. However, the polymerization of clickable vinyl monomers with a concurrent regulation on tacticity remains underdeveloped. Herein, we report the successful development of a stereoselective cationic copolymerization of C-C triple bond-containing vinyl ethers with simple alkyl vinyl ethers by employing confined Brønsted acid as catalyst, which allows for the synthesis of alkyne-functionalized vinyl ether copolymers with high isotacticity (up to 90 % m), controlled molecular weight, and variable content of C-C triple bonds.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!