Low temperature preparation of a graphene-cobalt microsphere hybrid by borohydride-initiated reduction for enriching proteins and peptides.

J Mater Chem B

School of Pharmacy, Department of Chemistry, Fudan University, 826 Zhangheng Road, Shanghai 201203, China.

Published: August 2014

A graphene-cobalt microsphere hybrid was prepared by the chemical reduction of a mixture containing graphene oxide and cobalt chloride. It was found that a little amount of potassium borohydride or sodium borohydride could initiate the hydrazine-reduction of the mixture at a low temperature of 80 °C. The structure of the material was investigated by scanning electron microscopy, transmission electron microscopy, energy dispersive spectroscopy, vibrating sample magnetometery, Fourier transform infrared spectroscopy, Raman spectroscopy, and Brunauer-Emmett-Teller (BET) techniques. The average size of the cobalt microspheres on graphene sheets was measured to be ∼590 nm. Magnetic investigations indicated that the graphene-cobalt microsphere hybrid exhibited ferromagnetic behavior at room temperature. In addition, the magnetic hybrid was successfully employed in the enrichment and identification of low-abundance proteins and peptides in combination with mass spectrometry.

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http://dx.doi.org/10.1039/c4tb00302kDOI Listing

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