CNC-Centre for Neuroscience and Cell Biology, UC Biotech, University of Coimbra, Lot 8A, Biocant Park, 3060-197, Cantanhede, Portugal; Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal. Electronic address:
Published: July 2020
AI Article Synopsis
Article Abstract
Mitochondria was used to clarify the effects of Coolia malayensis strain UNR-02 crude extract by studying mitochondrial membrane potential (ΔΨ) generation and the fluctuations of ΔΨ associated with the induction of mitochondrial permeability transition (MPT). The cytoxicity of C. malayensis was also determined using both HepG2 and H9c2(2-1) cells. C. malayensis extract significantly depressed the oxidative phosphorylation efficiency, as was inferred from the perturbations in ΔΨ and in the phosphorylative cycle induced by ADP. Increased susceptibility to Ca-induced MPT was also observed. At the cellular level, the extract significantly decreased cell mass of both cell lines.
Laboratório de Microalgas Marinhas, Universidade Federal do Estado do Rio de Janeiro - UNIRIO, Av. Pasteur, 458, Urca, Rio de Janeiro, 22290-240, RJ, Brazil. Electronic address:
Benthic dinoflagellates produce potent toxins that may negatively affect humans and the marine biota. Understanding the factors that stimulate their growth is important for management strategies and to reduce their potential negative impacts. Laboratory cultures have been extensively used to study microalgae physiology and characterize life cycles, nutrition, growth rates, among other processes.
Coolia species are diverse dinoflagellates found globally in tropical areas, and during a 2016 survey in Bahía Calderilla, one was identified in macroalgae samples.
The dinoflagellate was cultured and identified as C. malayensis through scanning electron microscopy and phylogenetic analysis, linking it to strains from New Zealand, Mexico, and the Asia Pacific.
While the cultured strain D005-1 didn't show detectable levels of known toxins, further research is needed to investigate its toxicity and potential effects in northern Chilean waters.
It is important to decipher the diversity and distribution of benthic dinoflagellates, as there are many morphologically indistinct taxa that differ from one another in production of potent toxins. To date, the genus Ostreopsis comprises twelve described species, of which seven are potentially toxic and produce compounds presenting a threat to human and environmental health. In this study, isolates previously identified as "Ostreopsis sp.
Coolia Meunier 1919 from benthic assemblages of Hawai'i and Guam were isolated and clonal cultures were established from single cells. Cultures were identified to species-level based on 28S rRNA and ITS-5.8S rRNA genes and tested for toxicity.
State Key Laboratory of Marine Pollution, City University of Hong Kong, Hong Kong, China; Research Centre for the Oceans and Human Health, City University of Hong Kong Shenzhen Research Institute, Shenzhen, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, China.
Coolia malayensis is one of the commonly found benthic dinoflagellates in Hong Kong which can produce biotoxins and threaten the early life stages of marine invertebrates. Seawater temperature has been recognized as one of the primary environmental factors that affect the formation of harmful algal blooms. The present study evaluated the responses of C.
