Antimicrobial Effect of a Peptide Containing Novel Oral Spray on .

Objective: To investigate the antibacterial effect of a novel antimicrobial peptide containing oral spray GERM CLEAN on and further explore the related mechanisms at phenotypic and transcriptional levels.

Methods: The disk diffusion method was used to preliminarily appraise the antimicrobial effect of GERM CLEAN. The minimal inhibitory concentration (MIC) of GREM CLEAN towards was determined by the broth dilution method. was determined by the broth dilution method.

Results: The diameter (10.18 ± 1.744 mm) of inhibition zones formed by GERM CLEAN preliminarily indicated its inhibitory effect on the major cariogenic bacteria was determined by the broth dilution method. was determined by the broth dilution method. was determined by the broth dilution method. was determined by the broth dilution method. , , , and were significantly repressed by treating with GERM CLEAN, and this was consistent with our phenotypic results.

Conclusion: The novel antimicrobial peptide containing oral spray GERM CLEAN has an anti- effect and the inhibitory property may be due to suppression of the virulence factors of including adhesive, acidogenicity, EPS, and biofilm formation. effect and the inhibitory property may be due to suppression of the virulence factors of including adhesive, acidogenicity, EPS, and biofilm formation. was determined by the broth dilution method.