Glucose isomerase (GI), an enzyme with deserved high potential in the world market. GI plays a major role in high Fructose Corn Syrup Production (HFCS). HFCS is used as a sweetener in food and pharmaceutical industries. are well-known producers of various industrially valuable enzymes, including Glucose isomerase. Currently, recombinant strains have been available for the production of various enzymes, but it has limitation in the large scale production. Therefore, identifying effective strains have emerged. The current study, the novel RSU26 was isolated from a marine source and optimized its potential to produce glucose isomerase at different physical and chemical conditions. The optimum pH and temperature for GI and biomass production were 7.5 and 35 °C, respectively at 96 h. Characterization study revealed that the approximate molar mass of GI was 43 kDa for monomeric and 170 kDa for tetrameric forms. Kinetic behavior exhibits , and values for the conversion of fructose to glucose conversion were 48.8 mM and 2.54 U mg at 50 °C and glucose to fructose were 29.4 mM and 2.38 U mg at 65 °C protein, respectively. Therefore, the present study suggested that the wild-type RSU26 has strong potential to produce glucose isomerase for various industrial applications.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105671 | PMC |
| http://dx.doi.org/10.1016/j.sjbs.2019.12.024 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Modulating the pH-activity profile of the glucose isomerase from Thermotoga marimita by introducing positively and negatively charged residues.
Biophys Chem
December 2024
School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266500, China.
Glucose isomerase is generally used in the industrial production of high-fructose corn syrup, and a heat- and acid-resistant glucose isomerase is preferred. However, most glucose isomerases exhibit low activity or inactivation at low pH. In this study, we demonstrated that two combination mutants formed by introducing positive and negative charges near the active site and on the surface of the enzyme demonstrated a successful reduction in the optimal pH and increase in the specific activity of glucose isomerase from Thermotoga maritima (TMGI).
View Article and Find Full Text PDFEngineering xylose utilization in Cupriavidus necator for enhanced poly(3-hydroxybutyrate) production from mixed sugars.
Bioresour Technol
December 2024
Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; Division of Energy and Environment Technology, KIST School, University of Science and Technology, Seoul 02792, Republic of Korea. Electronic address:
Lignocellulosic biomass is a promising renewable feedstock for biodegradable plastics like polyhydroxyalkanoates (PHAs). Cupriavidus necator, a versatile microbial host that synthesizes poly(3-hydroxybutyrate) (PHB), the most abundant type of PHA, has been studied to expand its carbon source utilization. Since C.
View Article and Find Full Text PDFContinuous Evolution of Protein through T7 RNA Polymerase-Guided Base Editing in .
ACS Synth Biol
December 2024
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
targeted mutagenesis technologies are the basis for the continuous directed evolution of specific proteins. Here, an efficient mutagenesis system (CgMutaT7) for continuous evolution of the targeted gene in was developed. First, cytosine deaminase and uracil-DNA glycosylase inhibitor were sequentially fused to T7 RNA polymerase using flexible linkers to build the CgMutaT7 system, which introduces mutations in targeted regions controlled by the T7 promoter.
View Article and Find Full Text PDFEfficient calcium fumarate overproduction from xylose and corncob-derived xylose by engineered strains of Aureobasidium pullulans var. Aubasidani DH177.
Microb Cell Fact
December 2024
College of Marine Life Sciences, Ocean University of China, Yushan Road, No. 5, Qingdao, China.
Article Synopsis
- Xylose, derived from lignocellulose, is a crucial renewable resource for producing valuable bioproducts like fumaric acid; optimizing its conversion is essential.
- The study identified a genetically modified strain (TKL-4) of A. pullulans that effectively uses xylose and corncob-derived xylose to produce calcium fumarate, demonstrating higher yields compared to glucose.
- The TKL-4 strain achieved impressive fermentation results, generating up to 88.5 g/L of calcium fumarate from xylose during a 10-liter fermentation process, showcasing its potential for eco-friendly bioproduct development.
Advances in the biosynthesis of D-allulose.
World J Microbiol Biotechnol
November 2024
School of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng, China.
Article Synopsis
- * There are two main ways to produce D-allulose: chemical synthesis, which can create unwanted byproducts, and biosynthesis, which uses enzymes to convert starch or glycerol into D-allulose more efficiently.
- * The article reviews recent research on biosynthesis, highlighting the enzymes used, their properties, and the potential for improved production methods for D-allulose.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!