The green synthesis of the flavor esters, -propyl acetate, isobutyl acetate and isoamyl acetate had the advantages over the chemical synthesis. The esterase from could transform -propanol, isobutanol and isoamyl alcohol into -propyl acetate, isobutyl acetate and isoamyl acetate, respectively. The esterase was expressed in . At 30 °C for 1 d, the concentration of -propyl acetate, isobutyl acetate and isoamyl acetate synthesized by the esterase expressed in was 24.6 mg/100 mL, 8.3 mg/100 mL, 5.6 mg/100 mL, respectively. Expression of the esterase has a practical significance for flavor ester synthesis by green biochemical process.
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http://dx.doi.org/10.1007/s12088-020-00856-9 | DOI Listing |
Cell Death Dis
January 2025
Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Lymphangiogenesis is vital for tissue fluid homeostasis, immune function, and lipid absorption. Abnormal lymphangiogenesis has been implicated in several diseases such as cancers, inflammatory, and autoimmune diseases. In this study, we elucidate the role of tsRNA-0032 in lymphangiogenesis and its molecular mechanism.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
February 2025
Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.
The cytoplasmic membrane of bacteria is composed of a phospholipid bilayer made up of a diverse set of lipids. Phosphatidylglycerol (PG) is one of the principal constituents and its production is essential for growth in many bacteria. All the enzymes required for PG biogenesis in have been identified and characterized decades ago.
View Article and Find Full Text PDFNucleic Acids Res
January 2025
Beijing Key Laboratory for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing 100083, China.
The flexibility and programmability of CRISPR-Cas technology have made it one of the most popular tools for biomarker diagnostics and gene regulation. Especially, the CRISPR-Cas12 system has shown exceptional clinical diagnosis and gene editing capabilities. Here, we discovered that although the top loop of the 5' handle of guide RNA can undergo central splitting, deactivating CRISPR-Cas12a, the segments can dramatically restore CRISPR function through nucleic acid self-assembly or interactions with small molecules and aptamers.
View Article and Find Full Text PDFPlant J
January 2025
Center for Legume Plant Genetics and Systems Biology, Oil Crops Research Institute, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Bacterial wilt caused by Ralstonia solanacearum is a devastating disease affecting a great many crops including peanut. The pathogen damages plants via secreting type Ш effector proteins (T3Es) into hosts for pathogenicity. Here, we characterized RipAU was among the most toxic effectors as ΔRipAU completely lost its pathogenicity to peanuts.
View Article and Find Full Text PDFJ Gastroenterol Hepatol
January 2025
Department of Gastroenterology, Songjiang Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Background: Hepatocellular carcinoma (HCC) and colorectal cancer (CRC) pose a significant threat to human health worldwide, characterized by intricate pathogenesis. A PC-esterase domain containing 1A (PCED1A) is a critical number of the GDSL/SGNH superfamily.
Aim: The aim of this study is to explore the diagnostic value of PCED1A in HCC and CRC and its relationship with immune infiltration.
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