A novel cellobiohydrolase (CBH)-generating fungi have been isolated and categorized as KMJ820 based on morphology and rDNA gene sequence. Cellulose powder was used as carbon source, the total enzyme activity was 11.51 U/ml is noted; which is among the highest amounts of CBH-generating microbes studied. CBH have been purified to homogenize, with pursual of serial chromatography using supernatants and two different CBHs were found; CBH 1 and 2. The filtered CBHs showed greater activity ( = 51.4 and 20.8 U/mg) in contrast to CBHs from earlier studies. The MW (molecular weights) of CBH 1 and 2 were verified to be approximately 50 kDa and 150 kDa, respectively, by size exclusion chromatography. Even though CBHs have been evaluated from other sources, but CBH is prominent in comparison to other CBHs by its high enzyme activity.
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| http://dx.doi.org/10.1007/s12088-019-00843-9 | DOI Listing |
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