Synchrotron radiolysis generates hydroxyl radicals (OH) that are successful footprinting reagents. Here, we describe a new reagent for the synchrotron platform, the trifluoromethyl radical (CF). The radical is produced by OH displacement of CF from sodium triflinate (Langlois reagent). Upon X-ray beam exposure, the reagent labels proteins extensively without any additional chemicals on a millisecond or shorter time scale. The CF is comparably reactive to OH and produces footprinting information that complements that of OH alone. This reagent in combination with OH should enable novel chemistry for protein footprinting on the synchrotron platform.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7486011 | PMC |
| http://dx.doi.org/10.1021/jasms.0c00085 | DOI Listing |
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