A self-assembled multivalent glycosidase inhibitor based on perylene bisimide-deoxynojirimycin conjugates was constructed, inhibited α-mannosidase and exhibited a K value of 38 nM, increased approximately 2763-fold compared with the control drug (miglitol). Furthermore, the postprandial blood glucose (PBG) level in mice of PBI-DNJ was firstly studied. PBI-DNJ exhibited a hypoglycaemic effect in vivo. Importantly, this work developed a new means to explore the hypoglycaemic effect in mice based on self-assembled glycosidase inhibitors.
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Article Synopsis
- * These structures can disassemble at different pH levels due to an ester linkage, and their ability to bind to lysozyme is influenced by the ratio of chitobiose in the mixture, showing higher binding affinity with increased chitobiose content.
- * The complex of chitobiose and lysozyme exhibits slower lytic activity compared to lysozyme alone, suggesting that while it binds strongly, it may delay the enzyme's effectiveness in breaking down bacteria.
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