RNA plays a central role in controlling cellular functions. Research of the content and distribution of RNA in living cells is of great significance to both biochemistry and biomedicine. However, ratiometric fluorescent probes for the detection of RNA in the cytoplasm and nucleoli are still rarely reported. We herein present the first example of a novel ratiometric fluorescent composite nanomaterial by using graphene quantum dots (GQDs) and a fluorescent probe molecule for the sensitive and selective detection of RNA. HVC-6 was selected as the detection group. The fluorescence was excited at 365 nm and the fluorescence emission at 470 and 610 nm increased gradually with the addition of RNA. The fluorescence intensity ratio of I/I displayed a linear response to RNA. Furthermore, the developed nanomaterial HVC-6@GQDs showed potential for utilization as a fluorescent RNA probe in living cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c8tb01125g | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Rhodamine-functionalized carbon dots with pH-regulated FRET efficiency for ratiometric fluorescence sensing and imaging of extremely alkaline pH.
Mikrochim Acta
January 2025
School of Pharmaceutical Science, Hengyang Medical School, University of South China, Hengyang, 421001, China.
A ratiometric fluorescent nanoprobe (CDs-Rho), synthesized through the simple covalent amide linkage between carbon dots (CDs) and pH-sensitive rhodamine dye (Rho), was designed for the precise sensing and imaging of extremely alkaline environments. The sensing mechanism involves the opposite pH-dependent fluorescence changes in CDs and Rho, respectively, coupled with pH-regulated FRET efficiency from CDs to Rho. The nanoprobe features a wide pH response window from pH 7.
View Article and Find Full Text PDFA ratiometric fluorescent probe for sensitively tracking peroxynitrite during drug-induced hepatotoxicity.
Chembiochem
January 2025
Xinzhou Normal University, Department of Chemistry, CHINA.
As one of the essential components of reactive oxygen species (ROS), peroxynitrite (ONOO-) plays an indispensable role in redox homeostasis and signal transduction processes, and its deviant levels are associated with numerous clinical diseases. Therefore, accurate and rapid detection of intracellular ONOO- levels is crucial for revealing its role in physiological and pathological processes. Herein, we constructed a ratiometric fluorescent probe to detect ONOO- levels in biological systems.
View Article and Find Full Text PDFFabrication of Dual-Ligand Zn-MOFs with Asynchronous Fluorescence Response for Efficient Ratiometric/Visual Sensing of Tetracycline Antibiotics in Animal-Derived Foods.
J Agric Food Chem
January 2025
College of Quality and Technical Supervision, Hebei University, Baoding 071002, China.
The extensive use of tetracyclines in livestock poses health risks due to their residues in animal-derived food; therefore, developing simple detection methods to replace complex traditional approaches is of paramount importance. Here, we developed a dual-ligand zinc-based metal-organic framework material, Zn-BTC-BDC-NH (denoted as ZTD), for the detection of tetracyclines. The intrinsic blue fluorescence of ZTD was quenched upon the introduction of tetracyclines due to electron transfer from -NH of ZTD to -CO- and -OH groups of tetracycline molecules; meanwhile, the new green fluorescence emission was generated through π-π stacking between aromatic rings and the formation of complexes between Zn and C-O/C═O groups.
View Article and Find Full Text PDFRatiometric fluorescent aptasensor based on DNA-gated FeO@Uio-66-NH and Exo I-assisted signal amplification.
Anal Chim Acta
February 2025
Food Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518045, PR China.
Background: Ochratoxin A (OTA) is toxic secondary metabolites produced by fungi and can pose a serious threat to food safety and human health. Due to the high stability and toxicity, OTA contamination in agricultural products is of great concern. Therefore, the development of a highly sensitive and reliable OTA detection method is crucial to ensure food safety.
View Article and Find Full Text PDFA Spectroscopic Methodology to Early Detection of Urinary Tract Infections.
Sensors (Basel)
January 2025
Centro de Química Estrutural, Institute of Molecular Sciences, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
Healthcare-associated infections (HAI) are a critical public health problem, with 30 to 40% of infections related to the urinary tract system. These urinary tract infections (UTIs) are considered one of the most common microbial infections in hospital settings and everyday community contexts, where approximately 80% are highly correlated with urinary catheter insertion, i.e.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!