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The lipid composition of yeast cells modulates the response to iron deficiency. | LitMetric

The lipid composition of yeast cells modulates the response to iron deficiency.

Biochim Biophys Acta Mol Cell Biol Lipids

Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (IATA), , Consejo Superior de Investigaciones Científicas (CSIC), Paterna, Valencia, Spain. Electronic address:

Published: August 2020

AI Article Synopsis

  • Iron is crucial for eukaryotes as it plays a key role in various metabolic processes, including lipid production.
  • Yeast cells missing the Mga2 transcription factor struggle to activate iron acquisition genes when iron is low, due to reduced levels of unsaturated fatty acids (UFAs).
  • Enhancing UFA levels or expressing the Ole1 gene can fix this issue, indicating that both Mga2 and Ole1 are necessary for proper iron regulation in response to deficiency.

Article Abstract

Iron is a vital micronutrient for all eukaryotes because it participates as a redox cofactor in multiple metabolic pathways, including lipid biosynthesis. In response to iron deficiency, the Saccharomyces cerevisiae iron-responsive transcription factor Aft1 accumulates in the nucleus and activates a set of genes that promote iron acquisition at the cell surface. In this study, we report that yeast cells lacking the transcription factor Mga2, which promotes the expression of the iron-dependent Δ9-fatty acid desaturase Ole1, display a defect in the activation of the iron regulon during the adaptation to iron limitation. Supplementation with exogenous unsaturated fatty acids (UFAs) or OLE1 expression rescues the iron regulon activation defect of mga2Δ cells. These observations and fatty acid measurements suggest that the mga2Δ defect in iron regulon expression is due to low UFA levels. Subcellular localization studies reveal that low UFAs cause a mislocalization of Aft1 protein to the vacuole upon iron deprivation that prevents its nuclear accumulation. These results indicate that Mga2 and Ole1 are essential to maintain the UFA levels required for Aft1-dependent activation of the iron regulon in response to iron deficiency, and directly connect the biosynthesis of fatty acids to the response to iron depletion.

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Source
http://dx.doi.org/10.1016/j.bbalip.2020.158707DOI Listing

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