AI Article Synopsis
- Nitric oxide (NO) is a key signaling molecule in various organisms, but its role and metabolism in yeast are not well understood due to difficulties in measuring its levels.
- A new method was developed to accurately measure NO in yeast cells, using an NO-specific fluorescence probe and high-performance liquid chromatography (HPLC/FLD), achieving a detection limit of 6 nM.
- The study found that fluorescence observed in yeast cells under certain stress conditions (like ethanol and heat) wasn't due to NO, demonstrating the effectiveness of this new measurement technique.
Article Abstract
Nitric oxide (NO) is an important signaling molecule involved in various biological phenomena in many organisms. The physiological functions and metabolism of NO in yeast, a unicellular microorganism, are still unknown, mainly because it is difficult to analyze the intracellular NO levels accurately. Here, we developed a new method of more accurately measuring NO content in yeast cells with the detection limit of 6 nM, by treating the cells with an NO-specific fluorescence probe followed by high-performance liquid chromatography with fluorescence detection (HPLC/FLD). This approach successfully detected and quantified the NO content inside yeast cells treated with an NO donor. Moreover, the HPLC/FLD analysis indicates that the fluorescence induced under some environmental stress conditions, such as ethanol, vanillin, and heat-shock, was not derived from NO. The HPLC/FLD method developed in this study provides a new strategy for measuring the intracellular NO concentration with higher accuracy.
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| http://dx.doi.org/10.1016/j.ab.2020.113707 | DOI Listing |
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