AI Article Synopsis
- DNA nanostructures are innovative materials made from DNA molecules that can create various nanoscale shapes, but conventional designs are limited by their dependence on low temperatures and magnesium for stability.
- This study introduces a new DNA nanonetwork, or "nanomesh," that is thermally stable and can function without magnesium, allowing it to maintain stability outside of solution environments.
- The nanomesh contains a significant amount of single-stranded DNA, enabling it to have biological functions and capabilities such as selectively adsorbing specific DNA molecules and enriching proteins, as demonstrated in preliminary experiments.
Article Abstract
DNA nanostructures are a new type of technology for constructing nanomaterials that has been developed in recent years. By relying on the complementary pairing of DNA molecules to form a double-stranded property, DNA molecules can construct a variety of nanoscale structures of 2D and 3D shapes. However, most of the previously reported DNA nanostructures rely solely on hydrogen bonds to maintain structural stability, resulting in DNA structures that can be maintained only at low temperature and in the presence of Mg, which greatly limits the application of DNA nanostructures. This study designed a DNA nanonetwork structure (nanonet) and changed its topological structure to DNA nanomesh by using DNA topoisomerase to make it thermally stable, while escaping the dependence on Mg, and the stability of the structure can be maintained in a nonsolution state. Moreover, the nanomesh also has a large amount of ssDNA (about 50%), providing active sites capable of exerting biological functions. Using the above characteristics, we prepared the nanomesh into a device capable of adsorbing specific DNA molecules, and used the device to enrich DNA. We also tried to mount antibodies using DNA probes. Preliminary results show that the DNA nanomesh also has the ability to enrich specific proteins.
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| http://dx.doi.org/10.1021/acsami.0c02242 | DOI Listing |
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