In this paper, a glutathione functionalized gold nanocluster (GSH-AuNCs) was prepared. GSH-AuNCs can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide to produce a blue-green ox-TMB. By using its peroxidase activity and the GSH-AuNCs-TMB-HO system, the visualization of Fe and Cu and the high sensitivity detection of Fe and Cu can be realized according to the change of absorbance value and color of the system. The results showed that the sensitivity of the system to detect Fe and Cu in industrial wastewater reached 1.25 × 10 M and 1.25 × 10M, respectively. At the same time, the chelating agents NHF and EDTA · 2Na were introduced to realize the selective detection of the two ions under the coexistence of Fe and Cu ions.
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