Following publication of the original article [1], it was brought to our attention of an error in the article title.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7118842 | PMC |
http://dx.doi.org/10.1186/s12871-020-00989-w | DOI Listing |
Anal Bioanal Chem
January 2025
Biospring Gesellschaft für Biotechnologie, Alt-Fechenheim 34, Frankfurt am Main, 60386, Germany.
The use of single-guide RNA (sgRNA) for gene editing using the CRISPR Cas9 system has become a powerful technique in various fields, especially with the growing interest in such molecules as therapeutic options in the last years. An important parameter for the use of these molecules is the verification of the correct sgRNA oligonucleotide sequence. Apart from next-generation sequencing protocols, mass spectrometry (MS) has been proven as a powerful technique for this purpose.
View Article and Find Full Text PDFBrain Res Bull
January 2025
Mental Health Center, the First Affiliated Hospital of Harbin Medical University, Heilongjiang, China. Electronic address:
Long noncoding RNA (lncRNA) are essential for modulating the onset and progression of alcohol use disorder (AUD). In this study, we investigated the molecular pathways through which lncRNA may contribute to AUD development. We assessed the expression levels of long noncoding RNA GAS5 (lncRNA GAS5) and microRNA-136-5p (miR-136-5p) in AUD tissue samples and cell lines using reverse transcription-quantitativepolymerase chain reaction.
View Article and Find Full Text PDFEnzyme Microb Technol
January 2025
Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Building 221, Technical University of Denmark, Lyngby DK-2800 Kgs, Denmark. Electronic address:
Aspergillus spp. and Rhizopus spp., used in solid-state plant food fermentations, encode cobalamin-independent methionine synthase activity (MetE, EC 2.
View Article and Find Full Text PDFNarra J
December 2024
Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Alzheimer's disease (AD) is the most frequent form of dementia and represents an increasing global burden, particularly in countries like Indonesia, where the population has begun to age significantly. Current medications, including cholinesterase inhibitors and NMDA receptor antagonists, have modest effects on clinical symptoms in the early to middle stages, but there is no curative treatment available so far despite progress. Activating or repressing epigenetic modifications, including DNA methylation, histone modification and microRNA regulation, appears to play an important role in AD development.
View Article and Find Full Text PDFJ Prosthodont Res
January 2025
Department of Comprehensive Dentistry, UT Health San Antonio, San Antonio, USA.
Purpose: To determine the effects of K18 quaternary ammonium methacryloxy silane (QAS) on tissue conditioner materials and their antimicrobial properties.
Methods: 30% K18 QAS in methyl methacrylate (MMA; K18-MMA; 0%, 15%, and 20% w/w) was incorporated into a commercial tissue conditioner (Coe comfort). The degree of curing (Shore A hardness), hydrophilicity (contact angle), flow, liquid sorption, mass loss, and antimicrobial properties of Streptococcus mutans, Streptococcus sanguinis, and Candida albicans were determined.
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!