The design of enzyme mimics from stable and nonprotein systems is especially attractive for applications in highly specific cancer diagnosis and treatment, and it has become an emerging field in recent years. Herein, metal crosslinked polymeric nanogels (MPGs) were prepared using Fe ion coordinated biocompatible acryloyl-lysine polymer brushes obtained from an enzyme-catalyzed atomic transfer radical polymerization (ATRPase) method. The monoatomic and highly dispersed Fe ions in the MPGs serve as efficient crosslinkers of the gel network, and also as active centers of multienzyme mimics of superoxide dismutase (SOD) and peroxidase (POD). The catalytic activities were compared to those of conventional Fe-based nanozymes. Studies on both cells and animals verify that efficient reactive oxygen species (ROS) responsive biofluorescence imaging can be successfully realized using the MPGs.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1002/anie.202002331 | DOI Listing |
Anal Chim Acta
January 2025
School of Forensic Medicine, China Medical University, No.77 Puhe Road, Shenyang, Liaoning, 110122, China. Electronic address:
The detection of biomarkers is crucial for assessing disease status and progression. Uric acid (UA), a common biomarker in body fluids, plays an important role in the diagnosis and monitoring of conditions such as hyperuricemia, chronic kidney disease, and cardiovascular disease. However, the low concentration of UA in non-invasive body fluids, combined with numerous interfering substances, makes its detection challenging.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
March 2025
National Engineering Laboratory for AIDS Vaccine, Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. Electronic address:
Nanozymes are nanoparticles with enzymatic activity, which are widely used in environmental and antibacterial research. Herein, we designed and synthesized novel amorphous nanozyme Cu-Im NPs with multiple enzyme-mimicking activities. Cu-Im NPs have the same active sites as natural laccase.
View Article and Find Full Text PDFColloids Surf B Biointerfaces
January 2025
Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital & Center for Molecular Imaging Probe & Hunan Engineering Research Center for Early Diagnosis and Treatment of Liver Cancer, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China. Electronic address:
The rational design of nanozymes with highly efficient reactive oxygen species (ROS) generation to overcome the resistant infection microenvironment still faces a significant challenge. Herein, the highly active Fe single-atom nanozymes (Fe SAzymes) with a hierarchically porous nanostructure were prepared through a colloidal silica-induced template method. The proposed Fe SAzymes with satisfactory oxidase (OD)-like and peroxidase (POD)-like activity can transform O and HO to superoxide anion free radical (•O) and hydroxyl radical (•OH), which possess an excellent bactericidal effect.
View Article and Find Full Text PDFTalanta
February 2025
Department of Chemistry, School of Science, China Pharmaceutical University, Nanjing, 211198, Jiangsu, PR China. Electronic address:
Designing innovative nanozymes with satisfactory catalytic activity and biocompatibility through simple and inexpensive approaches and excavating their multifunctional applications have crucial value and significance for the development of nanozyme science. In this work, RuNPs with excellent oxidase (OXD)-mimetic and peroxidase (POD)-like activity were synthesized via simple one-pot solvothermal reaction and exploited for disease marker detection, elimination of cancer cells, and in vitro antibacterial. Based on the OXD-mimetic activity of RuNPs, a new colorimetric biosensor was ingeniously designed for α-glucosidase (α-Glu) activity determination and screening of its inhibitors.
View Article and Find Full Text PDFSmall
December 2024
Capital Medical University, No. 10 Xitoutiao, You An Men, Beijing, 100069, China.
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!